Towards a genetic understanding of seed vigour in small-seeded crops using natural variation in Brassica oleracea
Towards a genetic understanding of seed vigour in small-seeded crops using natural variation in Brassica oleracea
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DOI：
10.1016/j.plantsci.2010.06.005
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发表时间：
2010-12-01
期刊：
影响因子：
5.2
通讯作者：
Morris, Karl
中科院分区：
文献类型：
--
作者：
Finch-Savage, William E.;Clay, Heather A.;Morris, Karl
育苗对于小种子作物的盈利和可持续生产至关重要。报告了一项工作方案，确定了可预测的幼苗建立所需的关键种子活力性状，然后进行定量遗传分析，以确定影响它们的基因座。三个关键特征是：（1）发芽快;（2）幼苗的快速初始向下生长;和（3）在增加阻抗的土壤中向上的芽生长的高潜力。所有这三个性状都缩短了播种和出苗之间的时间，而此时苗床可能会恶化。这表明了一种避免胁迫的策略，即在有足够水分的情况下通过快速进展，而不是依赖于胁迫耐受性。B。对甘蓝双单倍体作图群体进行了这些性状的筛选，并鉴定了数量性状基因座（QTL）。然后在可能的情况下对这些QTL进行精细定位。我们报告这项工作的结果，并讨论在其他QTL研究的背景下的结果。然后概述了标记和候选基因鉴定的前进方向，其使用翻译方法来利用存在于B之间的共线性。oleracea和模式植物拟南芥。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Seedling establishment is critical to profitable and sustainable production from small-seeded crops. A programme of work is reported that identifies key seed vigour traits that are required for predictable seedling establishment followed by a quantitative genetic analysis to identify loci that influence them. The three key traits were: (1) rapid germination; (2) rapid initial downward growth of the seedling; and (3) a high potential for upward shoot growth in soil of increasing impedance. All three traits reduce the time between sowing and seedling emergence when the seed bed can be deteriorating. This suggests a strategy of stress avoidance, through rapid progress when adequate moisture is present, rather than reliance on stress tolerance. B. oleracea doubled haploid mapping populations were screened for these traits and quantitative trait loci (QTL) were identified. These QTL were then subsequently fine-mapped where possible. We report on the outcome of this work and discuss the results in the context of other QTL studies. A way forward for marker and candidate gene identification is then outlined that uses a translational approach to exploit the colinearity that exists between B. oleracea and the model plant Arabidopsis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.