Comparative Transcriptome Analysis ofLitopenaeus vannameiReveals That Triosephosphate Isomerase-Like Genes Play an Important Role During Decapod Iridescent Virus 1 Infection
Comparative Transcriptome Analysis ofLitopenaeus vannameiReveals That Triosephosphate Isomerase-Like Genes Play an Important Role During Decapod Iridescent Virus 1 Infection
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凡纳滨对虾比较转录组分析揭示磷酸丙糖异构酶样基因在十足目虹彩病毒1型感染中的重要作用
DOI：
10.3389/fimmu.2020.01904
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发表时间：
2020-08-28
影响因子：
7.3
通讯作者：
Zhang, Shuang
中科院分区：
文献类型：
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作者：
Liao, Xuzheng;Wang, Chenggui;Zhang, Shuang
Decapod iridescent virus 1 (DIV1) results in severe economic losses in shrimp aquaculture. However, little is known about the physiological effect of DIV1 infection on the host. In this study, we found that the lethal dose 50 of DIV1-infectedLitopenaeus vannameiafter 48, 72, 96, and 156 h were 4.86 x 10(6), 5.07 x 10(5), 2.13 x 10(5), and 2.38 x 10(4)copies/mu g DNA, respectively. In order to investigate the mechanisms of DIV1 infection, a comparative transcriptome analysis of hemocytes fromL. vannamei, infected or not with DIV1, was conducted. The BUSCO analysis showed that the transcriptome was with high completeness (complete single-copy BUSCOs: 57.3%, complete duplicated BUSCOs: 41.1%, fragmentation: 0.8%, missing: 0.8%). A total of 168,854 unigenes were assembled, with an average length of 601 bp. Based on homology searches, Kyoto Encyclopedia of Genes and Genomes (KEGG), gene ontology (GO), and cluster of orthologous groups of proteins (KOG) analysis, 62,270 (36.88%) unigenes were annotated. Among them, 1,112 differentially expressed genes (DEGs) were identified, of which 889 genes were up-regulated and 223 genes were down-regulated after DIV1 infection. These genes were mainly annotated to the major metabolic processes such as fructose and mannose metabolism, carbon metabolism, and inositol phosphate metabolism. Among these metabolic pathways, the triosephosphate isomerase (TPI) family was the most eye-catching DEG as it participates in several metabolic processes. Three types ofTPI, LvTPI-like, LvTPI-Blike, andLvTPI-Blike1, were obtained for gene silencing by RNA interference. The results showed thatLvTPI-likeandLvTPI-Blike1silencing caused a high mortality rate amongL. vannamei. However,LvTPI-likeandLvTPI-Blikesilencing reduced DIV1 replication in DIV1-infectedL. vannamei. All the results indicated thatTPI-likegenes play an important role during DIV1 infection, which provides valuable insight into the infection mechanism of DIV1 in shrimp and may aid in preventing viral diseases in shrimp culture.
