Cartilage-inducing factor-A. Apparent identity to transforming growth factor-beta.
Cartilage-inducing factor-A. Apparent identity to transforming growth factor-beta.
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软骨诱导因子-A。
DOI：
10.1016/s0021-9258(17)38436-3
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Piez
中科院分区：
文献类型：
--
作者：
Saeid;M.;Seyedin;Andrea;Y.;Thompson;Hanne BentzS;David;RosenS;John;McPhersonS;A. Conti;Ned;R.;SiegelT;Gerald R Galluppi;Karl;A.;Piez
牛脱矿骨软骨诱导因子-A（CIF-A）N-末端30个氨基酸序列与人转化生长因子-β（TGF-β）相应序列的比较显示100%的同一性。此外，CIF-A刺激正常大鼠肾成纤维细胞成为锚定非依赖性和形成集落在软琼脂（在表皮生长因子的存在下）以类似于TGF-β的方式。类似地，来自人血小板的TGF-β诱导大鼠肌肉间充质细胞以与CIF-A等同的方式分化和合成软骨特异性大分子。这些数据表明，CIF-A和TGF-β是密切相关或相同的分子，并且这些因子可能参与细胞分化，包括软骨形成，作为软骨内骨形成的第一步。
Comparison of the sequence of the N-terminal 30 amino acids of cartilage-inducing factor-A (CIF-A) from bovine demineralized bone with the corresponding sequence of human transforming growth factor-beta (TGF-beta) revealed 100% identity. Furthermore, CIF-A stimulated normal rat kidney fibroblasts to become anchorage-independent and form colonies in soft agar (in the presence of epidermal growth factor) in a manner similar to TGF-beta. Similarly, TGF-beta from human platelets induced rat muscle mesenchymal cells to differentiate and synthesize cartilage-specific macromolecules in a manner equivalent to CIF-A. These data show that CIF-A and TGF-beta are closely related or identical molecules and that these factors may be involved in cell differentiation including cartilage formation as the first step in endochondral bone formation.