The membrane-bound rat serotonin transporter, SERT1, is an oligomeric protein
The membrane-bound rat serotonin transporter, SERT1, is an oligomeric protein
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DOI：
10.1016/0014-5793(96)00916-7
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发表时间：
1996-09-23
期刊：
影响因子：
3.5
通讯作者：
Schloss, P
中科院分区：
文献类型：
--
作者：
Jess, U;Betz, H;Schloss, P
神经递质5 -羟色胺的突触作用由位于轴突膜上的选择性再摄取系统终止。为了获得该膜蛋白的四级结构信息，我们在人胚胎肾293细胞中短暂表达了重组大鼠5 -羟色胺转运蛋白SERT1，巯基氧化剂和同源双功能交联剂二甲基亚酰咪啶（DMS）分别产生了130-180 kDa和220-270 kDa的加合物。这些数据表明SERT1具有低聚结构。
The synaptic actions of the neurotransmitter serotonin are terminated by a selective re-uptake system located in the axonal membrane, To gain information about the quaternary structure of this membrane protein, me transiently expressed the recombinant rat serotonin transporter, SERT1, in human embryonic kidney 293 cells, Treatment with sulfhydryl oxidizing agents and the homobifunctional cross-linker dimethyl suberimidate (DMS) generated adducts of 130-180 kDa and 220-270 kDa, respectively. These data indicate an oligomeric structure of SERT1.