NON-ALLELIC VARIANTS OF HISTONE-2A, HISTONE-2B AND HISTONE-3 IN MAMMALS
NON-ALLELIC VARIANTS OF HISTONE-2A, HISTONE-2B AND HISTONE-3 IN MAMMALS
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DOI：
10.1038/266273a0
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发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
ZWEIDLER, A
中科院分区：
文献类型：
--
作者：
FRANKLIN, SG;ZWEIDLER, A
真核生物的基因组由核蛋白纤维组成，这些核蛋白纤维是由 DNA 与小型碱性蛋白（组蛋白）相互作用以及某些组蛋白之间的特定相互作用形成的。为了更深入地了解染色体的分子组织，有必要详细确定组蛋白的复杂性和变异性。真核生物的染色体含有五种主要类型的组蛋白。其中之一，富含赖氨酸的 H1，由少量的多肽组成，这些多肽的一级结构略有不同，并且在不同组织中以不同的相对量存在。参与组蛋白-组蛋白相互作用的其他四种组蛋白被认为是同质且不变的，直到有可能将 H2a、H2b 和 H3 解析为在非离子去污剂存在下通过聚丙烯酰胺凝胶电泳显示出组织特异性变异的变体3（图 1）。我们在此报告哺乳动物组蛋白 2a、2b 和 3 变体的一级结构。
THE genome of eukaryotes is organised in nucleoprotein fibres formed by the interaction of the DNA with small basic proteins, the histones, and by specific interactions between certain histones1. To gain more insight into the molecular organisation of chromosomes it is necessary to determine in detail the complexity and variability of the histones. The chromosomes of eukaryotes contain five major types of histones. One of these, the very lysine-rich H1, consists of a small number of poly-peptides which differ slightly in primary structure and which are present in different relative amounts in different tissues2. The other four histones, which are involved in histone–histone interactions, were considered homogeneous and invariable until it became possible to resolve H2a, H2b and H3 into variants which exhibit tissue-specific variation by polyacrylamide gel electrophoresis in presence of non-ionic detergents3(Fig. 1). We report here on the primary structure of the variants of mammalian histones 2a, 2b and 3.