ROXAS - an efficient and accurate tool to detect vessels in diffuse-porous species
ROXAS - an efficient and accurate tool to detect vessels in diffuse-porous species
复制标题
ROXAS - 一种高效、准确的工具，用于检测散孔物种中的血管
DOI：
10.1163/22941932-00000034
复制
发表时间：
2013
期刊：
影响因子：
1.9
通讯作者：
B. Eilmann
中科院分区：
文献类型：
--
作者：
L. Wegner;G. Arx;U. Sass;B. Eilmann
木材解剖参数是研究过去树木生长限制的宝贵资料，是树木年代学和生态生理学之间的联系。然而，分析这些参数是一个耗时的过程，只有少数几个长年表存在。为了提高木材解剖参数的测量效率，开发了自动图像分析系统ROXAS等新型工具。到目前为止，ROXAS仅应用于环形多孔物种的大型早期血管的测量。在这项研究中，我们评估了ROXAS是否也适用于扩散多孔欧洲山毛榉的有效和准确的血管检测和测量。为此，我们将ROXAS的结果与既定测量程序Image-Pro Plus的结果在效率和准确性方面进行了比较。两种方法在效率上存在很大差异，使用ROXAS的自动测量速度比使用Image-Pro Plus快19倍。虽然这些方法导致了平均血管面积和血管密度的年变化模式相似，但绝对值不同。与ROXAS相比，Image-Pro Plus系统地测量了更低的平均血管面积和更高的血管密度。这归因于ROXAS中特定物种的技术设置，导致比使用Image-Pro Plus中的默认设置获得的结果更真实。然而，ROXAS的缺点是不能以足够的精度检测小血管（<100 μm²）。然而，根据欧洲山毛榉的薄片，通常很难区分这种小血管和薄壁细胞。此外，这些小容器对导电效率没有实质性的贡献。因此，如果要测量的容器面积下限设置为100 μm²，我们预计大多数研究不会出现任何问题。总的来说，ROXAS被证明对测量扩散多孔树种的血管参数是有用的，允许对大量样品进行准确和有效的分析。
Wood-anatomical parameters form a valuable archive to study past limitations on tree growth and act as a link between dendrochronology and ecophysiology. Yet, analysing these parameters is a time-consuming procedure and only few long chronologies exist. To increase measurement efficiency of wood-anatomical parameters, novel tools like the automated image-analysis system ROXAS were developed. So far, ROXAS has only been applied to measure large earlywood vessels in ring-porous species.In this study, we evaluate if ROXAS is also suitable for efficient and accurate detection and measurement of vessels in diffuse-porous European beech. To do so, we compared the outcome of ROXAS with that of the established measurement programme Image-Pro Plus in terms of efficiency and accuracy.The two methods differed substantially in efficiency with automatic measurements using ROXAS being 19 times faster than with Image-Pro Plus. Although the procedures led to similar patterns in annual variation of mean vessel area and vessel density, the absolute values differed. Image-Pro Plus measured systematically lower mean vessel areas and higher vessel densities than ROXAS. This was attributed to the species-specific technical settings in ROXAS, leading to more realistic results than those obtained using the default settings in Image-Pro Plus. A shortcoming of ROXAS was, however, that small vessels (<100 μm²) could not be detected with sufficient accuracy. Yet, based on thin sections of European beech, it is generally difficult to distinguish such small vessels from parenchyma cells. Moreover, these small vessels do not contribute substantially to conductive efficiency. Therefore, we do not foresee any problems for most studies if the lower vessel area threshold to be measured is set to 100 μm². Overall, ROXAS proved to be useful for measuring vessel parameters in diffuse-porous tree species, allowing accurate and efficient analyses of large numbers of samples.