Programmable Regulation of DNA Conjugation to Gold Nanoparticles via Strand Displacement
Programmable Regulation of DNA Conjugation to Gold Nanoparticles via Strand Displacement
复制标题
通过链置换对 DNA 与金纳米粒子缀合进行可编程调节
DOI：
10.1021/acs.langmuir.7b02620
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发表时间：
2017
期刊：
影响因子：
3.9
通讯作者：
Yang Jing
中科院分区：
文献类型：
--
作者：
Zhang Cheng;Wu Ranfeng;Li Yifan;Zhang Qiang;Yang Jing
最近，DNA与金纳米颗粒(AuNPs)的偶联方法引起了人们的极大关注。以可编程的方式控制这种结合的能力是人们非常感兴趣的。在这里，我们开发了一种基于逻辑的方法，通过级联DNA链置换来操纵硫代DNA物种与AuNPs的结合。利用这种方法，建立了多个基于逻辑的操作系统，并同时引入了多达三种DNA信号。此外，基于熵驱动的过程也实现了更灵敏的催化逻辑操作。在实验中，所有DNA/AuNPs的偶联结果都通过琼脂糖凝胶进行了验证。这种策略有望将DNA支架自动连接到无标记的金纳米粒子上，并可扩展到构建用于诊断、生物传感和分子机器人的DNA/纳米粒子设备。
Methods for conjugating DNA to gold nanoparticles (AuNPs) have recently attracted considerable attention. The ability to control such conjugation in a programmable way is of great interest. Here, we have developed a logic-based method for manipulating the conjugation of thiolated DNA species to AuNPs via cascading DNA strand displacement. Using this method, several logic-based operation systems are established and up to three kinds of DNA signals are introduced at the same time. In addition, a more sensitive catalytic logic-based operation is also achieved based on an entropy-driven process. In the experiment, all of the DNA/AuNPs conjugation results are verified by agrose gel. This strategy promises great potential for automatically conjugating DNA stands onto label-free gold nanoparticles and can be extended to constructing DNA/nanoparticle devices for applications in diagnostics, biosensing, and molecular robotics.