ISOLATION AND IDENTIFICATION OF HUMAN BRAIN NATRIURETIC PEPTIDES IN CARDIAC ATRIUM
ISOLATION AND IDENTIFICATION OF HUMAN BRAIN NATRIURETIC PEPTIDES IN CARDIAC ATRIUM
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DOI：
10.1016/0006-291x(90)92081-a
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发表时间：
1990-03-16
影响因子：
3.1
通讯作者：
MATSUO, H
中科院分区：
文献类型：
--
作者：
HINO, J;TATEYAMA, H;MATSUO, H
利用新建立的人脑钠素（BNP）放射免疫分析（RIA）系统，在心房中发现了高浓度的免疫反应性（ir-）人脑钠素（hBNP）。采用抗hbnp IgG免疫亲和层析和反相高效液相色谱（HPLC）技术，从心房提取物中分离出ir-hBNP的4K和13-15K两种分子形式。通过微测序，确定肽段为前hbnp （.gamma.- hbnp）及其c端32氨基酸肽（hBNP-32）。基于这些结果，在心房中，hBNP的加工途径与猪BNP （pBNP）相似，但与大鼠BNP不同，尽管低分子量hBNP-32是与pBNP相反的主要形式，而pBNP以高分子量。γ -pBNP的形式存在。
By using a radioimmunoassay (RIA) system newly established for human brain natriuretic peptide (BNP), a high concentration of immunoreactive (ir-) human BNP (hBNP) has been found in cardiac atrium. Two molecular forms of ir-hBNP of 4K and 13-15K were isolated from atrial extracts by using anti-hBNP IgG immunoaffinity chromatography and reverse phase high performance liquid chromatography (HPLC). By microsequencing, the peptides were determined to be a pro-hBNP (.gamma.-hBNP) and its C-terminal 32-amino acid peptide (hBNP-32). Based on these results, in cardiac atrium, hBNP is found to be processed in a pathway similar to that of porcine BNP (pBNP) but distinct from that of rat BNP, although low MW hBNP-32 is a major form in contrast to pBNP which exists as a high MW .gamma.-pBNP.