Sexual phenotype of avian chimeric gonads with germinal and stromal cells of opposite genetic sexes.
Sexual phenotype of avian chimeric gonads with germinal and stromal cells of opposite genetic sexes.
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具有相反遗传性别的生发细胞和基质细胞的鸟类嵌合性腺的性表型。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
F. Dieterlen
中科院分区：
文献类型：
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作者：
K. Hajji;C. Martin;A. Perramon;F. Dieterlen
在通过手术重组获得的嵌合性腺中，分析了生殖细胞和基质细胞在确定性腺的性表型中的各自作用。两个胚盘之间交换了相等的区域，以使生殖腺新月体和性腺区域具有不同的起源（图3）。用于这些实验的胚胎携带性连锁色素突变，这使得在性腺被取回进行检查时诊断生殖细胞和基质的遗传性别成为可能。根据种属的不同，采用了三种不同的组合方式：鸡或鹌鹑间质中的鸡生殖细胞，鸡间质中的鹌鹑生殖细胞。在每个嵌合体中，两个性腺细胞群体的遗传性别可以相同或相反。然而，生殖细胞群体似乎并不总是同质的。在某些嫁接方案中，异位生殖细胞，位于外生殖新月，有助于实验性腺的殖民化。这些生殖细胞与性腺的基质成分来自同一区域，即，它们是同一物种，具有相同的性别无论如何，在研究的87个嵌合体中，性腺的性别表型总是与基质的遗传性别相对应。因此，生殖细胞的遗传性别在性腺雏形的性别分化中没有作用。
The respective roles of germinal and stromal cells in determining the sexual phenotype of the gonad were analyzed in chimeric gonads obtained by surgical recombination between young avian blastodiscs in ovo. Equivalent territories were exchanged between two blastodisc, in order that the germinal crescent and the gonad territory had a different origin (fig. 3). Embryos used for these experiments carried a sex linked pigment mutation, that made it possible to diagnose the genetic sexes of germ cells and stroma at the time when the gonad was retrieved for examination. On the basis of species, three types of combination were performed: chick germ cells in chick or quail stroma, quail germ cells in chick stroma. In each chimera, the genetic sexes of the two gonadal cell populations could be identical or opposite. However it appeared that the germ cell population was not always homogeneous. In some grafting schemes, ectopic germ cells, located outside the germinal crescent, contributed to the colonization of the experimental gonad. These germ cells were from the same territory as the stroma element of the gonad, i.e., they were of the same species and the same genetic sex. Whatever the case, in 87 chimeras that were studied, the sex phenotype of the gonads always corresponded to the genetic sex of the stroma. Thus the genetic sex of germ cells has no role in the sexual differentiation of the gonadal rudiments.