Surface design with self-heating smart polymers for on-off switchable trap
Surface design with self-heating smart polymers for on-off switchable trap
复制标题
采用自加热智能聚合物的表面设计，可实现开关式疏水阀
DOI：
10.1088/1468-6996/12/4/044609
复制
发表时间：
2011
影响因子：
5.5
通讯作者：
P.Techawanitchai
中科院分区：
文献类型：
--
作者：
海法康裕;荒井陽一;鈴鴨よしみ;P.Techawanitchai
我们开发了一种新型的自热、温度响应型层析系统，用于有效分离生物分子。温度响应性聚(N-isopropylacrylamide-co-N-hydroxymethylacrylamide)，聚(NIPAAm-co-HMAAm)以“开-关”可切换的表面陷阱的形式共价接枝到磁铁矿/二氧化硅复合材料的表面。通过改变HMAAm的含量，将聚(NIPAAm-co-HMAAm)S的低临界溶解温度控制在35~55℃。利用磁性粒子在交变磁场(AMF)中产生的热，我们能够诱导接枝的温度响应性聚合物从亲水相分离到疏水相分离。为了评价聚(NIPAAm-co-HMAAm)接枝磁铁矿/二氧化硅微粒作为固定相用于层析的可行性，我们将这些微粒装入高效液相色谱系统的玻璃柱中，并分析了类固醇的逃逸图谱。疏水类固醇在AMF中的保留时间显著增加，这是因为接枝的磁铁矿/二氧化硅粒子通过自加热增强了疏水相互作用，这种作用可以通过改变AMF的照射时间来控制。关闭AMF可以缩短类固醇的总分析时间。该系统可用于生物活性化合物的分离，因为AMF可以很容易地控制它们的洗脱曲线。
We have developed a novel self-heating, temperature-responsive chromatography system for the effective separation of biomolecules. Temperature-responsive poly (N-isopropylacrylamide-co-N-hydroxymethylacrylamide), poly (NIPAAm-co-HMAAm), was covalently grafted onto the surface of magnetite/silica composites as' on-off'switchable surface traps. The lower critical solution temperature (LCST) of the poly (NIPAAm-co-HMAAm) s was controlled from 35 to 55 C by varying the HMAAm content. Using the heat generated by magnetic particles in an alternating magnetic field (AMF) we were able to induce the hydrophilic to hydrophobic phase separation of the grafted temperature-responsive polymers. To assess the feasibility of the poly (NIPAAm-co-HMAAm)-grafted magnetite/silica particles as the stationary phase for chromatography, we packed the particles into the glass column of a liquid chromatography system and analyzed the elusion profiles for steroids. The retention time for hydrophobic steroids markedly increased in the AMF, because the hydrophobic interaction was enhanced via self-heating of the grafted magnetite/silica particles, and this effect could be controlled by changing the AMF irradiation time. Turning off the AMF shortened the total analysis time for steroids. The proposed system is useful for separating bioactive compounds because their elution profiles can be easily controlled by an AMF.