Induction of migration of periodontal ligament cells by selective regulation of integrin subunits
Induction of migration of periodontal ligament cells by selective regulation of integrin subunits
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通过选择性调节整合素亚基诱导牙周膜细胞迁移
DOI：
10.1111/jcmm.14023
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发表时间：
2019
影响因子：
5.3
通讯作者：
Kawamura Mari，Yamamoto Tadashi，Yamashiro Keisuke，Kochi Shinsuke，Yoshihara‐Hirata Chiaki，Ideguchi Hidetaka，Aoyagi Hiroaki，Omori Kazuhiro，Takashiba Shogo .
中科院分区：
文献类型：
--
作者：
中尾 雄紀,福田 隆男;讃井 彰一;田中 麗;渡邊 ゆかり、大和 寛明、四本 かれん、西村 英紀,;Kawamura Mari，Yamamoto Tadashi，Yamashiro Keisuke，Kochi Shinsuke，Yoshihara‐Hirata Chiaki，Ideguchi Hidetaka，Aoyagi Hiroaki，Omori Kazuhiro，Takashiba Shogo .
组织驻留干细胞的募集对创面再生非常重要。牙周韧带细胞(PDL细胞)是一种具有干性特征的异质细胞群，可迁移到创面以再生牙周纤维和邻近的硬组织。细胞迁移受局部微环境、生长因子和细胞外基质(ECM)的调节。整合素介导的细胞与细胞外基质的黏附为细胞迁移提供了必要的信号。我们假设整合素的选择性表达可以促进PDL细胞的迁移。用血小板衍生生长因子-BB(PDGF-BB)诱导原代培养的PDL细胞迁移。根据在迁移过程中观察到的整合素表达谱，研究了阻断特定整合素对细胞迁移和ECM黏附的影响。整合素α3、α5和纤维连接蛋白在迁移中的PDL细胞中的不同位置被检测到上调。经抗整合素α5抗体处理后，细胞迁移受到抑制。抗整合素α3、α3封闭肽和α3siRNA处理显著促进细胞迁移，与PDGFBB处理相当。此外，整合素α3抑制通过整合素α5优先增强与纤维连接蛋白的黏附。这些结果表明，整合素α3介导的抑制和α5介导的促进相互调节细胞的迁移。因此，靶向整合素表达是牙周再生的一种可能的治疗策略。
The recruitment of tissue‐resident stem cells is important for wound regeneration. Periodontal ligament cells (PDL cells) are heterogeneous cell populations with stemness features that migrate into wound sites to regenerate periodontal fibres and neighbouring hard tissues. Cell migration is regulated by the local microenvironment, coordinated by growth factors and the extracellular matrix (ECM). Integrin‐mediated cell adhesion to the ECM provides essential signals for migration. We hypothesized that PDL cell migration could be enhanced by selective expression of integrins. The migration of primary cultured PDL cells was induced by platelet‐derived growth factor‐BB (PDGF‐BB). The effects of blocking specific integrins on migration and ECM adhesion were investigated based on the integrin expression profiles observed during migration. Up‐regulation of integrins α3, α5, and fibronectin was identified at distinct localizations in migrating PDL cells. Treatment with anti‐integrin α5 antibodies inhibited PDL cell migration. Treatment with anti‐integrin α3, α3‐blocking peptide, and α3 siRNA significantly enhanced cell migration, comparable to treatment with PDGF‐BB. Furthermore, integrin α3 inhibition preferentially enhanced adhesion to fibronectin via integrin α5. These findings indicate that PDL cell migration is reciprocally regulated by integrin α3‐mediated inhibition and α5‐mediated promotion. Thus, targeting integrin expression is a possible therapeutic strategy for periodontal regeneration.
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纤连蛋白，原纤维生成和体内功能。
影响因子：
7.2
作者：
Schwarzbauer JE;DeSimone DW
通讯作者：
DeSimone DW
牙周组织中细胞粘附蛋白和整合素的免疫组织学定位。
影响因子：
4.3
作者：
Steffensen,B;Duong,AH;Milam,SB;Potempa,CL;Winborn,WB;Magnuson,VL;Chen,D;Zardeneta,G;Klebe,RJ
通讯作者：
Klebe,RJ
影响因子：
4.3
作者：
A. Takemura;I. Nakagawa;S. Kawai;H. Inaba;Takahiro Kato;S. Hamada;A. Amano
通讯作者：
A. Takemura;I. Nakagawa;S. Kawai;H. Inaba;Takahiro Kato;S. Hamada;A. Amano
影响因子：
4.1
作者：
Tadashi Yamamoto;Yuki Ugawa;M. Kawamura;K. Yamashiro;Shinsuke Kochi;Hidetaka Ideguchi;S. Takashiba
通讯作者：
Tadashi Yamamoto;Yuki Ugawa;M. Kawamura;K. Yamashiro;Shinsuke Kochi;Hidetaka Ideguchi;S. Takashiba
影响因子：
4.6
作者：
Joy T. Yang;H. Rayburn;R. Hynes
通讯作者：
Joy T. Yang;H. Rayburn;R. Hynes