Gravitational unloading inhibits the regenerative potential of atrophied soleus muscle in mice
Gravitational unloading inhibits the regenerative potential of atrophied soleus muscle in mice
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DOI：
10.1111/j.1748-1716.2008.01943.x
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发表时间：
2009-07-01
影响因子：
6.3
通讯作者：
Yoshioka, T.
中科院分区：
文献类型：
--
作者：
Matsuba, Y.;Goto, K.;Yoshioka, T.
本研究旨在探讨卸载对萎缩和损伤的骨骼肌再生的影响。使用8周龄的雄性小鼠(C57BL/6J)。将心脏毒素（CTX）注射到双侧比目鱼肌中。一组在注射CTX前2周和注射后6周通过后肢悬吊对比目鱼肌进行重力卸载。其余组中的比目鱼肌被加载，将小鼠关在笼子中，并在注射后14、28和42天进行解剖。CTX注射组中比目鱼肌的湿重和蛋白质含量的恢复受到卸载的抑制。 CTX 注射和加载诱导的卫星细胞数量的增加也受到卸载的抑制。单核细胞浸润到坏死区域的消失也被延迟。这种现象表明，再生（以具有中央核的纤维的出现为标志）受到卸载的抑制。结果表明，负载在激活受伤骨骼肌的再生潜力中起着重要作用。
The present study was performed to investigate the influence of unloading on the regeneration of atrophied and injured skeletal muscle.Male mice (C57BL/6J), aged 8 weeks, were used. Cardiotoxin (CTX) was injected into soleus muscles bilaterally. Gravitational unloading on soleus muscle was performed by hind limb suspension for 2 weeks before and additionally 6 weeks after CTX injection in one group. Soleus muscles in the remaining groups were loaded keeping the mice in the cages and were dissected 14, 28 and 42 days after the injection.Recovery of the wet weight and protein content of soleus in the CTX-injected group was inhibited by unloading. Increase in satellite cell number, induced by CTX injection and loading, was also inhibited by unloading. Disappearance of infiltration of mononucleated cells into the necrotic area was also delayed. This phenomenon suggests that regeneration, which is indicated by the appearance of fibres with central nuclei, was inhibited by unloading.Results suggested that loading plays an important role in the activation of the regenerating potential of injured skeletal muscle.