Beta-amyloid peptide-induced modifications in α7 nicotinic acetylcholine receptor immunoreactivity in the hippocampus of the rat:: Relationship with GABAergic and calcium-binding proteins perikarya
Beta-amyloid peptide-induced modifications in α7 nicotinic acetylcholine receptor immunoreactivity in the hippocampus of the rat:: Relationship with GABAergic and calcium-binding proteins perikarya
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DOI：
10.1016/j.brainresbull.2007.09.003
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发表时间：
2008-03-28
影响因子：
3.8
通讯作者：
Gonzalo-Ruiz, A.
中科院分区：
文献类型：
--
作者：
Arevalo-Serrano, J.;Sanz-Anquela, J. M.;Gonzalo-Ruiz, A.
在大鼠中研究了注射β-淀粉样蛋白（AP）对海马中烟碱乙酰胆碱受体蛋白（α 7 nAChR）α 7亚型的影响。注射AP到压后皮质导致海马中α 7 nAChR免疫反应性降低。定量分析显示，与未处理的对照动物的相应半球相比，A β注射侧同侧的CA 1背侧部分的α 7 nAChR免疫反应性显著降低，并且与对侧半球（对应于对照（PBS）注射侧）中观察到的结果一致。与同侧CA 1、CA 2和CA 3的腹侧部分相比，同侧CA 1的背侧部分的α 7 nAChR免疫反应性也显著降低。该分析还揭示了与未处理的对照动物的相应半球相比，A β注射侧同侧的齿状回中α 7 nAChR免疫反应性显著降低，并且与PBS注射侧中的情况相同。共定位研究表明α 7 nAChR蛋白高度定位于GABA和Parv免疫反应性细胞中，而只有少数Calb阳性细胞表达α 7 nAChR。此外，注射AP蛋白导致同侧CA 1的背侧部分中的GABA-和Parv-免疫反应细胞的数量显著减少，与未处理的对照动物的相应区域相比，并且与PBS注射侧的相应区域相比。我们的研究结果表明，AP诱导减少α 7 nAChR-含有细胞，这可能有助于在海马GABA能突触传递的损害。(C)2007爱思唯尔公司All rights reserved.
The effects of the injected beta-amyloid (AP) protein on the alpha 7 subtype of nicotinic acetylcholine receptor protein (alpha 7nAChR) in the hippocampus were studied in rats. Injections of AP into the retrosplenial cortex resulted in a decrease in alpha 7nAChR-immunoreactivity in the hippocampus. Quantitative analysis revealed a significant reduction in (alpha 7nAChR-immunoreactivity in the dorsal part of the CA1 ipsilateral to the A beta-injected side as compared to the corresponding hemisphere of non-treated control animals and with that seen in the contralateral hemisphere, which corresponds to the control (PBS)-injected side. A significant decrease in alpha 7nAChR-immunoreactivity was also found in the dorsal part of the ipsilateral CA1 as compared with that in the ventral part of the CA1, in CA2, and in CA3 ipsilateral to the A beta-injected side. The analysis also revealed a significant decrease in (alpha 7nAChR-immunoreactivity in the dentate gyrus ipsilateral to the A beta-injected side as compared to the corresponding hemisphere of non-treated control animals and with that in the PBS-injected side co-localization studies showed that the alpha 7nAChR protein is highly localized in GABA- and Parv-immunoreactive cells, while only few Calb-positive cells expressed immunoreactivity for alpha 7nAChR. In addition, injections of AP protein resulted in a significant reduction in the number of GABA- and Parv-immunoreactive cells in the dorsal part of the ipsilateral CA1 as compared to the corresponding region of non-treated control animals and with that in the corresponding region of the PBS-injected side. Our findings suggest that AP induces a reduction in alpha 7nAChR-containing cells, which may contribute to impairment of GABAergic synaptic transmission in the hippocampus. (C) 2007 Elsevier Inc. All rights reserved.