Interleukin-2 promotes the motility of dendritic cells and their accumulation in lung and skin
Interleukin-2 promotes the motility of dendritic cells and their accumulation in lung and skin
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DOI：
10.1159/000164033
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发表时间：
1996-07-01
期刊：
影响因子：
5
通讯作者：
Pinto, C
中科院分区：
文献类型：
--
作者：
Kradin, RL;Xia, WJ;Pinto, C
树突状细胞（DC）作为体内的抗原提呈细胞发挥着重要作用。先前已经表明，DC响应于IFN-γ的肠胃外注射而在肺中积累。在本文中，我们报告了大鼠DC表达白细胞介素-2受体（IL-2 R）α链（OX-39），并在Boyden室和视频延时显微镜测定中显示对IL-2的响应增强的运动性。用OX-39（抗IL-2 R）而不是OX-22（抗CD 45 RC）（同种型匹配的鼠单克隆抗体）预处理DC可特异性抑制运动性增加。肺内注射IL-2可增加肺小静脉周围和肺小叶内OX-6+树突状细胞的数量。当IL-2注射到大鼠足垫时。24 h真皮微静脉周围DC增多。这种作用被胃肠外注射抗OX-39阻断。我们的结论是，IL-2是一个有效的增强DC运动，并可能与其他辅助性T细胞（h）-1促炎细胞因子在吸引DC的炎症部位。
Dendritic cells (DC) play a critical role as antigen-presenting cells in vivo. It has previously been shown that DC accumulate in the lung in response to parenteral injections of IFN-gamma. In the current paper, we report that rat DC express the interleukin-2 receptor (IL-2R) alpha-chain (OX-39) and display enhanced motility in response to IL-2 in Boyden chamber and video time-lapse microscopy assays. Increased motility was specifically inhibited by pretreating DC with OX-39 (anti-IL-2R) but not OX-22 (anti-CD45RC), an isotype-matched murine monoclonal antibody. The intratracheal injection of IL-2 increased the number of OX-6+ dendritic cells located around pulmonary venules and in the lung interstitium. When IL-2 was injected into the footpad of the rat. DC were increased around dermal venules at 24 h. This effect was blocked by the parenteral injection of anti-OX-39. We conclude that IL-2 is a potent enhancer of DC motility and may cooperate with other T helper(h)-1 proinflammatory cytokines in attracting DC to sites of inflammation.