Protection and immune modulation of activated human vaginal epithelial cells by Aurea helianthus extract
Protection and immune modulation of activated human vaginal epithelial cells by Aurea helianthus extract
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DOI：
10.1038/s41598-020-65821-4
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发表时间：
2020-06-08
影响因子：
4.6
通讯作者：
Lee, SeungGwan
中科院分区：
文献类型：
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作者：
Park, Yoonjin;Lee, Kyunghwa;Lee, SeungGwan
向日葵提取物具有多种特性，包括抗黑色素生成、抗氧化、抑制肿瘤发生和免疫调节；然而，它执行人阴道上皮细胞（HVEC）免疫调节的机制仍然难以捉摸。我们确定了提取物的三种免疫功能。首先，它介导 HVEC 的致瘤抑制。在氧化和真菌应激下暴露于提取物的 HVEC 中，细胞角蛋白 8、癌抗原 125 和波形蛋白的表达显着下调。其次，提取物激活树突状细胞和巨噬细胞。将祖树突细胞暴露于提取物后，CD304(+) 细胞的数量增加了 40%；此外，在氧化和真菌应激下，这一数字比受到应激的细胞分别低约 1.8 和 1.3 倍。在单核细胞分化过程中，与对照组相比，树突状细胞和巨噬细胞的数量分别增加了9倍和6倍。此外，提取物增强和恢复极化的能力分别比压力条件下的约 1.5 倍和 2 倍。第三，巨噬细胞对HPV16、18、33肽的吞噬活性比应激条件下增强12-35倍。因此，向日葵提取物是免疫系统的强刺激剂，并能在应激条件下抑制肿瘤发生。
Aurea helianthus extract is associated with various properties including anti-melanogenesis, anti-oxidation, tumorigenic suppression, and immunoregulation; however, the mechanism by which it executes the immunomodulation of human vaginal epithelial cells (HVECs) remains elusive. We established three immunological functions of the extract. First, it mediated tumorigenic suppression in HVECs. Expression of cytokeratin 8, cancer antigen-125, and vimentin was dramatically downregulated in HVECs exposed to the extract under oxidative and fungal stresses. Second, the extract activated dendritic cells and macrophages. On exposing progenitor dendritic cells to the extract, the number of CD304(+) cells increased by 40%; further, under oxidative and fungal stresses, this number was approximately 1.8 and 1.3 times lower, respectively, compared to that in the stressed cells. In monocytic differentiation, the number of dendritic cells and macrophages increased 9 and 6 times, respectively, compared to that in the control. Additionally, the extract enhanced and recovered polarisation by approximately 1.5 and 2 times, respectively, than that under stressed conditions. Third, the phagocytic activity of macrophages, against HPV16, 18, and 33 peptides, was enhanced by 12-35 times compared with that under stressed conditions. Thus, A. helianthus extract is a strong stimulator of the immune system and tumorigenic suppression under stress conditions.