DEFINITION OF A CORE REGION OF RAG-2 THAT IS FUNCTIONAL IN V(D)J RECOMBINATION
DEFINITION OF A CORE REGION OF RAG-2 THAT IS FUNCTIONAL IN V(D)J RECOMBINATION
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DOI：
10.1093/nar/22.10.1805
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发表时间：
1994-05-25
影响因子：
14.9
通讯作者：
GELLERT, M
中科院分区：
文献类型：
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作者：
SADOFSKY, MJ;HESSE, JE;GELLERT, M
RAG-1和RAG-2基因的产物协同作用，使V(D)J在淋巴和非淋巴细胞中重组。作为了解RAG-2作用的一个步骤，我们构建了突变的RAG-2基因，并检测了它们在成纤维细胞系中支持质粒底物重组的能力。这些突变定义了RAG-2基因的基本部分和非必要部分。N端部分的突变几乎消除了所有的活性。在蛋白质的中心区域，一些但不是所有的局部改变仍然允许重组。另一方面，从C末端大量缺失的蛋白质，包括被截断25%的蛋白质，仍然保持活性，即使这部分蛋白质在物种之间高度保守。对于保留信号连接或编码连接或执行反转的底物，也获得了类似的结果。因此，V(D)J连接的所有基本特征都保留在RAG-2蛋白中，只有前75%的序列。
The products of the RAG-1 and RAG-2 genes cooperate to allow V(D)J recombination in lymphoid and non-lymphoid cells. As one step toward understanding the role of RAG-2, we have constructed mutated RAG-2 genes and examined their ability to support recombination of plasmid substrates in a fibroblast cell line. The mutations define essential and dispensable parts of the RAG-2 gene. Mutations in the N-terminal part eliminate almost all activity. In the central region of the protein, some but not all local alterations still allow recombination. On the other hand, proteins with large deletions from the C-terminal end, including one truncated by 25%, still retain activity, even though this part of the protein is highly conserved between species. Similar results were obtained with substrates that retain either a signal joint or a coding joint, or perform an inversion. Thus all basic features of V(D)J joining are retained in a RAG-2 protein with only the first 75% of the sequence.