CONDENSATION OF NUCLEIC-ACIDS BY INTERCALATING AROMATIC CATIONS
CONDENSATION OF NUCLEIC-ACIDS BY INTERCALATING AROMATIC CATIONS
复制标题
DOI：
10.1073/pnas.81.23.7368
复制
发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
DARZYNKIEWICZ, Z
中科院分区：
文献类型：
--
作者：
KAPUSCINSKI, J;DARZYNKIEWICZ, Z
某些嵌入的芳香族阳离子，如荧光染料吖啶橙子或抗肿瘤药物Miltoxantrone [1，4-dihydroxy-5，8-bis[[2-[（2-hydroxyethyl）amino]ethyl]amino]-9，10-anthracenedione]，诱导溶液中核酸的缩合。在用这些嵌入配体滴定核酸的过程中，可以通过光散射测量来定量监测凝聚形式的外观。所得的高度可再现的光散射过渡曲线是典型的合作过程，并且过渡发生在不同的配体临界浓度下，这取决于配体本身和核酸的一级结构（碱基和糖组成）和二级结构（单链或双链）。根据嵌入剂与单链核酸相互作用的模型，讨论了嵌入阳离子配体引起核酸缩合的机理，并与多价简单阳离子如Co ~（3+）或精胺~（4+）引起的缩合进行了比较。所描述的现象可以在分析和制备生物化学中用于表征核酸的一级和二级结构以及用于分离化合物。的可能性，缩合起作用的芳香族阳离子的诱变和药理作用被认为是。
Certain intercalating aromatic cations, such as the fluorochrome acridine orange or the antitumor drug Miltoxantrone [1,4-dihydroxy-5,8-bis[[2-[(2-hydroxyethyl)amino]ethyl]amino]-9,10-anthracenedione], induce condensation of nucleic acids in solutions. The apperance of the condensed form during titration of nucleic acids with these intercalating ligands can be quantitatively monitored by light scatter measurements. The resulting highly reproducible light scatter transition curves are typical of the cooperative processes, and the transitions occur at different critical concentrations of the ligands depending upon both the ligand itself and the primary structure (base and sugar composition) and the secondary structure (single- or double-stranded) of the nucleic acids. The mechanism of condensation of nucleic acids by intercalating cationic ligands is discussed in light of the model of interactions occurring between certain intercalators and single-stranded nucleic acids and compared with the condensation induced by polyvalent simple cations such as Co3+ or spermine4+. The described phenomenon can have an application in analytical and preparative biochemistry for characterization of the primary and secondary structure of nucleic acids and for separation of the compounds. The possibility that the condensation plays a role in mutagenic and pharmacological effects of aromatic cations is considered.