Cell wall polysaccharides of Brassica campestris seed cake:: isolation and structural features
Cell wall polysaccharides of Brassica campestris seed cake:: isolation and structural features
复制标题
DOI：
10.1016/j.carbpol.2004.01.012
复制
发表时间：
2004-08-12
影响因子：
11.2
通讯作者：
Ray, B
中科院分区：
文献类型：
--
作者：
Ghosh, P;Ghosal, P;Ray, B
甘蓝型油菜籽饼是水溶性多糖的重要来源。酸碱提取的多糖含量为270 mg/g脱脂粉。这些含有大量中性糖的聚合物是阿拉伯糖、鼠李半乳糖苷I和阿拉伯半乳糖蛋白。用尺寸排阻色谱对聚合物进行了分析。发现了两个重叠的峰。采用特异性酶解、离子交换层析(HPAEC)和基质辅助激光解吸电离飞行时间质谱仪对4M KOH可溶半纤维素多糖的结构进行了表征，结果表明芥末粕木聚糖为XXXG型，含有XXXG、XXFG、XXLG和XLFG(按Fry等人命名)。[PhysIOL.种。89(1993)1])作为主要建筑附属单位。内切-β-(1--)-D-D-木聚糖酶的水解和木聚糖寡糖的分析表明，木糖(27%)、木二糖(26%)和含有4-O-甲基-D-葡萄糖醛酸残基的酸性木聚糖片段(47%)存在。(C)2004爱思唯尔有限公司。保留所有权利。
Brassica campestris seed cake is an important source of water-soluble polysaccharides. The polysaccharides extracted with acid and dilute alkali represented 270 mg/g of defatted meal. These polymers, which have high amount of neutral sugars are arabinan, rhamnogalacturonan I and arabinogalactan proteins. These polymers have been analyzed by size exclusion chromatography. Two overlapping peaks have been found. Structural characterization of 4 M KOH-soluble hemicellulosic polysaccharides using specific enzyme hydrolysis, ion exchange chromatography (HPAEC) and matrix-assisted laser desorption ionization-time of flight mass spectroscopy showed that mustard meal xyloglucan is of XXXG type and contained XXXG, XXFG, XXLG and XLFG (named according to Fry et al. [Physiol. Plant. 89 (1993) 1]) as the major building sub-units. Hydrolysis with endo-beta-(1 --> 4)-D-xylanase and analysis of the xylan derived oligosaccharides showed the presence of xylose (27%), xylobiose (26%) and acidic xylan fragments containing 4-O-methyl-D-glucuronic acid residue (47%). (C) 2004 Elsevier Ltd. All rights reserved.