Designing polymer matrix for microchip-based double-stranded DNA capillary electrophoresis.
Designing polymer matrix for microchip-based double-stranded DNA capillary electrophoresis.
复制标题
DOI：
10.1016/j.chroma.2006.03.106
复制
发表时间：
2006-06
期刊：
影响因子：
--
通讯作者：
Jun Zhang;Weidong He;D. Liang;D. Fang;B. Chu;M. Gassmann
中科院分区：
文献类型：
--
作者：
Jun Zhang;Weidong He;D. Liang;D. Fang;B. Chu;M. Gassmann
Please try later.
Polyacrylamide (PAM) was used as a model polymer to build up an empirical model that relates polymer molecular weight, polymer concentration and solution viscosity. The desired random copolymers of acrylamide (AM) and N,N-dimethylacrylamide (DMA) used as DNA separation media for different specifications were synthesized under the guidance of the empirical model. The separation performances of rationally designed copolymers were tested in a 1.2cm long separation channel, simulating microchip-based capillary electrophoresis. pBR322/HaeIII digest was successfully separated with good separation resolution and fast speed. Validation of the sieving ability of our polymers was performed in the Agilent 2100 Bioanalyzer. The results of the 10bp (base pair) DNA ladder separation demonstrate the potential of our approach for the sieving matrix in microchip-based electrophoresis.