Surfactant Imprinting Hyperactivated Immobilized Lipase as Efficient Biocatalyst for Biodiesel Production from Waste Cooking Oil
Surfactant Imprinting Hyperactivated Immobilized Lipase as Efficient Biocatalyst for Biodiesel Production from Waste Cooking Oil
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表面活性剂印迹高活性固定化脂肪酶作为废食用油生产生物柴油的高效生物催化剂
DOI：
10.3390/catal9110914
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发表时间：
2019-11
期刊：
影响因子：
3.9
通讯作者：
Weiwei Zhang
中科院分区：
文献类型：
--
作者：
Huixia Yang;Weiwei Zhang
采用表面活性剂印迹法活化洋葱伯克霍尔德菌脂肪酶（BCL），并将其固定在磁性交联酶聚集体（mCLEAs）中，制备了羟基磷灰石包覆磁性纳米颗粒（HAP-coated MNP）。在用0.1 mM Triton X-100预处理BCL时观察到BCL mCLEAs的最大超活化。优化的Triton活化的BCL mCLEAs作为一种高活性和强大的生物催化剂用于从WCO生产生物柴油，表现出显着增加生物柴油产量和耐受甲醇。结果表明，整合mCLEA的表面活性剂印迹可以将BCL固定在其活性（开放）形式，经历活性的提高，并且允许在溶剂中进行生物柴油生产而无需进一步添加水。在优化的反应条件下，当甲醇与异丁醇的摩尔比为7：1，在40 °C的正己烷中一次投加时，生物柴油的产率可达98%。因此，本研究显示了一种通用的方法固定化脂肪酶，并显示了巨大的实用潜力，在可再生生物柴油的生产。
Enzymatic production of biodiesel from waste cooking oil (WCO) could contribute to resolving the problems of energy demand and environment pollutions.In the present work, Burkholderia cepacia lipase (BCL) was activated by surfactant imprinting, and subsequently immobilized in magnetic cross-linked enzyme aggregates (mCLEAs) with hydroxyapatite coated magnetic nanoparticles (HAP-coated MNPs). The maximum hyperactivation of BCL mCLEAs was observed in the pretreatment of BCL with 0.1 mM Triton X-100. The optimized Triton-activated BCL mCLEAs was used as a highly active and robust biocatalyst for biodiesel production from WCO, exhibiting significant increase in biodiesel yield and tolerance to methanol. The results indicated that surfactant imprinting integrating mCLEAs could fix BCL in their active (open) form, experiencing a boost in activity and allowing biodiesel production performed in solvent without further addition of water. A maximal biodiesel yield of 98% was achieved under optimized conditions with molar ratio of methanol-to-WCO 7:1 in one-time addition in hexane at 40 °C. Therefore, the present study displays a versatile method for lipase immobilization and shows great practical latency in renewable biodiesel production.
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DOI：
10.1016/j.ijbiomac.2019.08.246
发表时间：
2019-12
影响因子：
8.2
作者：
S. Kannan;M. Marudhamuthu
通讯作者：
S. Kannan;M. Marudhamuthu
DOI：
10.3390/molecules22122157
发表时间：
2017-12-07
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Zhang W;Yang H;Liu W;Wang N;Yu X
通讯作者：
Yu X
影响因子：
2.9
作者：
Barbe, Sophie;Lafaquiere, Vincent;Andre, Isabelle
通讯作者：
Andre, Isabelle
影响因子：
11.4
作者：
Dong, Tao;Zhao, Lin;Tan, Xin
通讯作者：
Tan, Xin
将脂肪酶共价固定在氨丙基功能化羟基磷灰石封装的 γ-Fe2O3 纳米粒子上：用于大豆油酯交换的磁性生物催化剂
影响因子：
8.8
作者：
Xie, Wenlei;Zang, Xuezhen
通讯作者：
Zang, Xuezhen