Combination of cytoplasmic and nuclear girdin expression is an independent prognosis factor of breast cancer
Combination of cytoplasmic and nuclear girdin expression is an independent prognosis factor of breast cancer
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DOI：
10.1096/fj.201700825rr
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发表时间：
2017-12
期刊：
影响因子：
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通讯作者：
Huikun Zhang;F. Yu;Fengxia Qin;Y. Shao;Wei Chong;Zhifang Guo;Xiaoli Liu;L. Fu;Feng Gu;Yongjie Ma
中科院分区：
文献类型：
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作者：
Huikun Zhang;F. Yu;Fengxia Qin;Y. Shao;Wei Chong;Zhifang Guo;Xiaoli Liu;L. Fu;Feng Gu;Yongjie Ma
Girdin是一种肌动蛋白结合蛋白，在各种癌症的发展中发挥关键作用。虽然在线工具预测了girdin的核定位概率很高，但迄今为止很少有令人信服的证据。本研究的目的是发现girdin的精确的亚细胞分布和潜在的预后价值相应的定位。通过临床病理分析，检测了Girdin在人乳腺癌细胞系和800多例人乳腺组织中的亚细胞分布。在本研究中，我们首次发现girdin可以附着在染色质上，并与细胞核中的拓扑异构酶-IIα相互作用。胞浆和核girdin在乳腺癌预后中表现出不同的作用：胞浆girdin表达是无进展生存期（PFS）的独立预后因素，而核girdin表达是总生存期（OS）的独立预后因素。更重要的是，细胞质和细胞核的结合是OS和PFS的独立预后因素。总之，我们的研究结果强烈建议细胞质和核girdin的组合分析，以准确预测乳腺癌的预后。张洪，余，F.，秦，F.，Shao，Y.，美国，Chong，W.，郭志，刘，X.，富湖，加-地顾，F.，马，Y。胞浆和胞核结合表达是乳腺癌的独立预后因素。FASEB J. 32，2395-2410（2018）。www.fasebj.org
Girdin is an actin‐binding protein playing key roles in the development of various carcinomas. Although online tools have predicted nuclear localization of girdin with a high probability, convincing proof has rarely been provided until now. The purpose of this study was to discover girdin's precise subcellular distribution and the potential prognostic value corresponding to its localization. The subcellular distribution of girdin was detected in a human breast cancer cell line and in >800 samples of human breast tissue by clinical pathologic analysis. In this study, we discovered for the first time that girdin could attach to chromatin and interact with topoisomerase‐IIα in nucleus. Cytoplasmic and nuclear girdin exhibited different roles in prognosis of breast cancer: cytoplasmic girdin expression was an independent prognostic factor for progression‐free survival (PFS), whereas nuclear girdin expression was an independent prognostic factor for overall survival (OS). More important, combination cytoplasmic and nuclear girdin was an independent prognosis factor of both OS and PFS. In conclusion, our research results strongly recommend combination analysis of cytoplasmic and nuclear girdin for a precise prognostic prediction in breast cancer.—Zhang, H., Yu, F., Qin, F., Shao, Y., Chong, W., Guo, Z., Liu, X., Fu, L., Gu, F., Ma, Y. Combination of cytoplasmic and nuclear girdin expression is an independent prognosis factor of breast cancer. FASEB J. 32, 2395–2410 (2018). www.fasebj.org