Arabidopsis FAB1A/B is possibly involved in the recycling of auxin transporters
Arabidopsis FAB1A/B is possibly involved in the recycling of auxin transporters
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DOI：
10.4161/psb.6.4.15023
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发表时间：
2011-01-01
影响因子：
2.9
通讯作者：
Sato, Masa H.
中科院分区：
文献类型：
--
作者：
Hirano, Tomoko;Sato, Masa H.
FAB1/PIKfyve由磷脂酰肌醇-3-磷酸(PtdIns 3-P)合成磷脂酰肌醇-3，5-二磷酸(PtdIns(3，5)P-2)，它不仅参与液泡/溶酶体的动态平衡，还参与将各种蛋白质运输到液泡或利用真核细胞中的内体回收质膜上的蛋白质(PM)。我们先前已经证明，拟南芥FAB1A/B在拟南芥和裂殖酵母中都具有PtdIns-3，5-激酶的功能，并且在空泡酸化和内吞作用中起着关键作用。虽然FAB1A/B条件基因敲除突变体对膜不通透性生长素2，4-二氯苯氧基乙酸(2，4-D)表现出生长素抗性表型，但对膜通透性人工生长素1-萘乙酸(NAA)没有这种表型。对2，4-D和NAA的敏感性差异与生长素抗性突变体(如AUX1)相似。综上所述，这些结果表明，拟南芥FAB1A/B功能的受损可能导致生长素转运体的膜循环能力缺陷，并抑制生长素向细胞内的正确运输。
Fab1/PIKfyve produces Phosphatidylinositol- 3,5-bisphosphate (PtdIns (3,5) P-2) from Phosphatidylinositol-3phosphate (PtdIns 3-P), and is involved not only in vacuole/lysosome homeostasis, but also in transporting various proteins to the vacuole or recycling proteins on the plasma membrane (PM) through the use of endosomes in a variety of eukaryotic cells. We previously demonstrated that Arabidopsis FAB1A/B functions as PtdIns-3,5-kinase in both Arabidopsis and fission yeast and plays a key role in vacuolar acidification and endocytosis. Although the conditional FAB1A/B knockdown mutant revealed an auxin-resistant phenotype to a membrane-impermeable auxin, 2,4-dichlorophenoxyacetic acid (2,4-D), the mutant did not exhibit this phenotype to a membrane-permeable artificial auxin, naphthalene 1-acetic acid (NAA). The difference in the sensitivities to 2,4-D and NAA is similar to those of the auxin-resistant mutants such as aux1. Taken together, these results suggest that impairment of the function of Arabidopsis FAB1A/B might cause a defect in the membrane recycling capabilities of the auxin transporters and inhibit proper auxin transport into the cells in Arabidopsis.