Comparative Analysis of Extracellular and Intracellular Proteomes of Listeria monocytogenes Strains Reveals a Correlation between Protein Expression and Serovar
Comparative Analysis of Extracellular and Intracellular Proteomes of Listeria monocytogenes Strains Reveals a Correlation between Protein Expression and Serovar
复制标题
DOI：
10.1128/aem.00594-08
复制
发表时间：
2008-12-01
影响因子：
4.4
通讯作者：
Hebraud, Michel
中科院分区：
文献类型：
--
作者：
Dumas, Emilie;Meunier, Bruno;Hebraud, Michel
单核细胞增生性李斯特菌（Listeria monocytogenes）是李斯特菌病的病原体，其在食品环境中的频繁发生以及高死亡率仍然是一个严重的公共卫生问题。在13个血清型中，人类淋病主要与血清型4 b、1/2b和1/2a菌株相关。探讨L.单核细胞增多症，12株菌的细胞内和细胞外蛋白进行了分析，通过双向凝胶电泳。这些菌株来源不同，分属不同血清型（4 b、1/2a和1/2b），在鸡胚中表现出不同的毒力水平。根据蛋白质组学模式将菌株分为两类，与L。单核细胞增生系统谱系。统计分析不允许识别的分离物的来源或菌株的毒力水平的特定的蛋白质，但26和21蛋白点被证明是显着过表达和低表达，分别在6株血清型1/2a（谱系II）相比，菌株的血清型1/2b或4 b。此外，青霉素结合蛋白是特异性的血清型1/2b和两个蛋白质点确定为丝氨酸蛋白酶特异性血清型4 b。这些蛋白质点，确定通过肽质量指纹图谱使用基质辅助激光解吸电离飞行时间质谱，基本上是在细胞外蛋白质组中发现的，并可能作为潜在的标记物用于血清分型和风险分析。
Listeria monocytogenes, the etiologic agent of listeriosis, remains a serious public health concern, with its frequent occurrence in food environments coupled with a high mortality rate. Among the 13 serovars, human listeriosis is mostly associated with the serovar 4b, 1/2b, and 1/2a strains. To investigate the diversity of L. monocytogenes, the intracellular and extracellular proteins of 12 strains were analyzed by two-dimensional gel electrophoresis. These strains had different origins, belonged to different serovars (4b, 1/2a, and 1/2b), and presented with different levels of virulence in chicken embryos. The clustering of the strains in two groups based on proteomic patterns is in agreement with the L. monocytogenes phylogenetic lineages. Statistical analysis did not allow for identification of proteins specific to the isolate origin or the virulence level of the strains, but 26 and 21 protein spots were shown to be significantly overexpressed and underexpressed, respectively, in the six strains of serovar 1/2a (lineage II) compared to strains of serovar 1/2b or 4b. Moreover, a penicillin-binding protein was specific for serovar 1/2b and two protein spots identified as a serine protease were specific to serovar 4b. These protein spots, identified through peptide mass fingerprinting using matrix-assisted laser desorption ionization-time of flight mass spectrometry, were essentially found in the extracellular proteome and may have uses as potential markers for serotyping and risk analysis.