Vibrational Dynamics in Photoactive Yellow Protein Revealed by Mid-IR Pump/Visible Probe Spectroscopy
Vibrational Dynamics in Photoactive Yellow Protein Revealed by Mid-IR Pump/Visible Probe Spectroscopy
复制标题
中红外泵/可见光探针光谱揭示光敏黄色蛋白的振动动力学
DOI：
10.1007/978-3-319-13242-6_128
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
濱田格雄
中科院分区：
文献类型：
--
作者：
中村亮介;濱田格雄
利用中红外泵浦/可见光探测光谱研究了光敏黄蛋白的振动动力学。激发发色团的振动模式后，在发色团中获得时间常数为0.2-0.3和5-7 ps的振动弛豫。另一方面，当泵浦脉冲与蛋白质骨架的C=O伸缩模式共振时，观察到具有4.3 ps时间常数的额外上升分量，这表明振动能量从蛋白质骨架流向发色团。
Vibrational dynamics in photoactive yellow protein is studied by mid-IR pump/visible probe spectroscopy. Vibrational relaxation with time constants of 0.2–0.3 and 5–7 ps in the chromophore are obtained upon excitation of the vibrational modes of the chromophore. On the other hand, when the pump pulse is resonant to the C=O stretching mode of the protein backbone, an additional rise component with a 4.3 ps time constant is observed, which indicates that the vibrational energy flows from the protein backbone to the chromophore.