Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies
Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies
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DOI：
10.1128/jb.00315-11
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发表时间：
2011-09-01
影响因子：
3.2
通讯作者：
Bernhardt, Thomas G.
中科院分区：
文献类型：
--
作者：
Dinh, Thuy;Bernhardt, Thomas G.
绿色荧光蛋白(GFP)的出口问题阻碍了对周质蛋白亚细胞定位的研究。在这里，我们证明了GFP的超折叠变体(SfGFP)是SEC介导的转运后的荧光，并且当使用该途径的共翻译分支时效果最好。
Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed.