Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis
Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis
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DOI：
10.1590/s1517-83822009000200009
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发表时间：
2009-06-01
影响因子：
2.2
通讯作者：
Savasan, Serap
中科院分区：
文献类型：
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作者：
Ciftci, Alper;Findik, Arzu;Savasan, Serap
本研究旨在检测耐甲氧西林和产生黏液的金黄色葡萄球菌在牛乳腺炎的情况下。优化了以16S rRNA、nuc和mecA基因为目标的三重PCR，分别用于葡萄球菌、金黄色葡萄球菌和甲氧西林耐药的检测。此外，对产泥菌株进行了icaA和icaD基因的PCR检测。本研究共检出59株金黄色葡萄球菌，其中耐甲氧西林13株，mecA基因阳性4株（30.7%）。虽然59株金黄色葡萄球菌中有22株（37.2%）在刚果红琼脂中产生黏液，但PCR分析中只有15株icaA和icaD基因均阳性。59株中icaA基因和icaD基因分别阳性16株和38株。59株菌株中只有2株对甲氧西林耐药和产黏液均呈阳性，这表明这些菌株对甲氧西林耐药和产黏液之间缺乏相关性。综上所述，本研究优化的三重PCR可快速、可靠地检测耐甲氧西林金黄色葡萄球菌。此外，仅以icaA和icaD为靶点的PCR可能不足以检测出产黏液，为了准确评价金黄色葡萄球菌菌株的产黏液特性，需要进一步以其他ica基因为靶点的研究。
This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains were positive for icaA and icaD gene, respectively. Only 2 of 59 strains were positive for both methicillin resistance and slime producing, phenotypically, suggesting lack of correlation between methicillin resistance and slime production in these isolates. In conclusion, the optimized triplex PCR in this study was useful for rapid and reliable detection of methicillin resistant S. aureus. Furthermore, only PCR targetting icaA and icaD may not sufficient to detect slime production and further studies targetting other ica genes should be conducted for accurate evaluation of slime production characters of S. aureus strains.