FUNGAL ELICITOR-MEDIATED RESPONSES IN PINE CELL-CULTURES .1. INDUCTION OF PHENYLPROPANOID METABOLISM
FUNGAL ELICITOR-MEDIATED RESPONSES IN PINE CELL-CULTURES .1. INDUCTION OF PHENYLPROPANOID METABOLISM
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DOI：
10.1007/bf00195322
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发表时间：
1992-02-01
期刊：
影响因子：
4.3
通讯作者：
ELLIS, BE
中科院分区：
文献类型：
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作者：
CAMPBELL, MM;ELLIS, BE
建立了一种组织培养系统来检测外生菌根共生体在松木组织中诱导的苯丙素代谢。外生菌根真菌Thelephora terrestris Fr.的激发子对松柏悬浮培养细胞的酚代谢有促进作用。通过组织木质素化和细胞中特定甲醇可提取化合物的积累表明。早在诱导后12 h就观察到木质素化的诱导。苯丙氨酸解氨酶（PAL, EC 4.3.1.5）是苯丙氨酸代谢的入口酶，其活性也在同一时间内升高。诱导后6小时PAL活性明显增加，诱导后12小时，诱导细胞的PAL活性比相应对照高10倍。诱导组织的木质素化还伴随着与木质素合成相关的其他酶的活性增加，包括咖啡酸o -甲基转移酶（EC 2.1.1.46），羟基肉桂酸：辅酶a连接酶（EC 6.2.1.12），肉桂醇脱氢酶（EC 1.1.1）。-)，松柏素-葡萄糖苷酶（EC 3.2.1.21）和过氧化物酶（EC 1.11.1.7）。总过氧化物酶活性的增加与可溶性过氧化物酶亚型的变化有关。松树细胞培养-外生菌根激发子体系为分析具有重要经济价值的针叶树种木质素化过程提供了良好的分子模型。
A tissue culture system has been developed to examine phenylpropanoid metabolism induced in pine tissues by an ectomycorrhizal symbiont. An elicitor preparation from the ectomycorrhizal fungus Thelephora terrestris Fr. induced enhanced phenolic metabolism in suspension cultured cells of Pinus banksiana Lamb., as indicated by tissue lignification and accumulation of specific methanol-extractable compounds in the cells. Induction of lignification was observed as early as 12 h after elicitation. The activity of phenylalanine ammonia-lyase (PAL, EC 4.3.1.5), the entry-point enzyme into phenylpropanoid metabolism, also increased within the same time-frame in elicited cells. Significant increases in PAL activity were evident by 6 h after elicitation, and, by 12 h after elicitation, PAL activity in elicited cells was ten times greater than that in the corresponding controls. Lignification of the elicited tissue was also accompanied by an increase in the activity of other enzymes associated with lignin synthesis, including caffeic acid O-methyl transferase (EC 2.1.1.46), hydroxycinnamate:CoA ligase (EC 6.2.1.12), cinnamyl alcohol dehydrogenase (EC 1.1.1.-), coniferin beta-glucosidase (EC 3.2.1.21) and peroxidase (EC 1.11.1.7). The increase in total peroxidase activity was associated with a change in the pattern of soluble peroxidase isoforms. The pine cell culture-ectomycorrhizal elicitor system provides a good model for molecular analysis of the process of lignification in an economically important softwood species.