Lectin histochemistry of developing submandibular glands of fetal Syrian golden hamsters.
Lectin histochemistry of developing submandibular glands of fetal Syrian golden hamsters.
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胎儿叙利亚金仓鼠下颌下腺发育的凝集素组织化学。
DOI：
10.1007/bf00174394
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
McDowell,EM
中科院分区：
文献类型：
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作者：
Ito,K;Ito,T;Strum,JM;McDowell,EM
凝集素结合研究了胎儿叙利亚金黄地鼠（Mesocricetus auratus）从妊娠第12天至16天（出生当天）发育中的颌下腺。将胎儿固定，石蜡包埋，切片，并用9种凝集素-辣根过氧化物酶结合物：刀豆球蛋白A（ConA）、麦胚凝集素（WGA）、双花扁豆凝集素（DBA）、苹果凝集素（HPA）、苹果凝集素（MPA）、番茄凝集素I-B4（GSA I-B4）、花生凝集素（PNA）、荆豆凝集素I（UEA I）和多汁鲎凝集素（LPA）染色。发育中的腺体表现出戏剧性的形态学变化，每天的基础上，伴随着凝集素染色的进行性变化。在第12天，原始腺体仅显示用WGA、HPA、MPA、PNA和UEA I进行的痕量凝集素染色，但到第13天，在导管腔形成后，在导管腔表面处观察到用这些凝集素以及DBA进行的强染色。第14天原始腺泡细胞中首次出现分泌颗粒，分泌产物用WGA、DBA、HPA、MPA、PNA和UEA I染色。在第15天，分泌产物也被GSA I-B4中度染色。第16天进一步分化为分泌细胞，但不同凝集素的粘蛋白的染色强度在分泌细胞中不同。在整个观察期间，LPA未能染色腺体的任何部分，Con A仅染色糖原。
Lectin binding was studied in the developing submandibular glands of fetal Syrian golden hamsters (Mesocricetus auratus) from gestational day 12 to 16 (the day of birth). The fetuses were fixed, embedded in paraffin, sectioned and stained with nine lectin-horseradish peroxidase conjugates: concanavalin A (Con A), wheat germ agglutinin (WGA),Dolichos biflorusagglutinin (DBA),Helix pomatiaagglutinin (HPA),Maclura pomiferaagglutinin (MPA),Griffonia simplicifoliaagglutinin I-B4(GSA I-B4), peanut agglutinin (PNA),Ulex europensagglutinin I (UEA I) andLimulus polyphemusagglutinin (LPA). The developing glands showed dramatic morphological alterations on a daily basis, accompanied by progressive changes in lectin staining. On day 12 the primitive gland showed only trace lectin staining with WGA, HPA, MPA, PNA and UEA I, but by day 13, strong staining with these lectins, as well as with DBA, was seen at the ductal lumenal surface, after the formation of the ductal lumens. Secretory granules first appeared in cells of the primitive acini on day 14; the secretion products were stained strongly with WGA, DBA, HPA, MPA, PNA and UEA I. On day 15, the secretion products were also stained moderately with GSA I-B4. Secretory differentiation was further developed on day 16, but the staining intensity of the mucins with the different lectins varied among the secretory cells. LPA failed to stain any part of the gland throughout the observation period, and Con A stained only glycogen.