Expression of calnexin reflects Paneth cell differentiation and function
Expression of calnexin reflects Paneth cell differentiation and function
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DOI：
10.1097/01.lab.0000041709.42598.4a
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发表时间：
2002-12-01
影响因子：
5
通讯作者：
Autschbach, F
中科院分区：
文献类型：
--
作者：
Gassler, N;Schnölzer, M;Autschbach, F
研究表明，Paneth细胞在化生过程中的行为和功能与正常肠黏膜不同。在这项研究中，我们研究了calnexin，一种参与分泌途径的蛋白质，是否可能与正常小肠Paneth细胞的分化和功能有关，在胃的完全肠化生和富含Paneth细胞的腺瘤中。通过Ki67、溶菌酶和形态学特征监测Paneth细胞的分化和功能。利用新建立的单克隆抗体，我们发现正常小肠Paneth细胞有规律地合成calnexin。在这些细胞中，calnexin的染色强度与其分泌颗粒（溶菌酶）的含量呈负相关。相比之下，肠化生Paneth细胞和富Paneth细胞腺瘤的calnexin和溶菌酶的免疫染色均降低。此外，这些Paneth细胞合成了增殖标志物Ki67，这一现象在正常小肠Paneth细胞中从未观察到。体外实验表明，calnexin的表达不受有丝分裂诱导的直接影响。综上所述，钙连联素可能反映了Paneth细胞的分化和功能状况。结果并不一定表明calnexin在Paneth细胞增殖中起作用。
It has been suggested that the behavior and function of Paneth cells in metaplasia are different from those found in normal intestinal mucosa. In this study, we investigated whether calnexin, a protein involved in secretory pathways, might be associated with differentiation and function of Paneth cells in normal small intestine, in complete intestinal metaplasia of the stomach, and in Paneth cell-rich adenomas. Differentiation and function of Paneth cells was monitored by Ki67, lysozyme, and morphologic features. Using a newly established monoclonal antibody, we found that calnexin is regularly synthesized by Paneth cells of normal small intestine. In these cells, the staining intensity of calnexin was inversely correlated with their content of secretory granules (lysozyme). In contrast, Paneth cells of intestinal metaplasia and Paneth cell-rich adenomas showed a reduced immunostaining of both calnexin and lysozyme. Moreover, these Paneth cells synthesized the proliferation marker Ki67, a phenomenon that was never observed in Paneth cells of normal small intestine. In vitro experiments using CaCo2 cells showed that the expression of calnexin is not directly affected by the induction of mitosis. In conclusion, calnexin probably reflects the status of Paneth cell differentiation and function. The results do not necessarily indicate that calnexin has a function in Paneth cell proliferation.