Energy transport from mitochondria to myofibril by a creatine phosphate shuttle in cardiac cells.
Energy transport from mitochondria to myofibril by a creatine phosphate shuttle in cardiac cells.
复制标题
通过心肌细胞中的磷酸肌酸穿梭从线粒体到肌原纤维的能量传输。
DOI：
10.1152/ajpcell.1983.245.5.c423
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Winegrad,S
中科院分区：
文献类型：
--
作者：
McClellan,G;Weisberg,A;Winegrad,S
在高渗透性心肌细胞中，其表面膜对小分子和离子具有高度渗透性，当ATP浓度福尔斯200 μ M时，静息张力增加。峰值静息张力发生在10 μ M ATP中，等于5mM ATP中最大Ca激活力的60%。如果提供O2、底物、ADP和无机磷酸盐（Pi），高渗透性细胞中的线粒体可以保持ATP浓度高于200 μ M。只要磷酸肌酸存在，从浴溶液中去除ATP不会增加静息张力。然而，在缺乏ATP和磷酸肌酸的情况下，O2、底物和Pi不能降低静息张力。这些结果被解释为腺嘌呤核苷酸与肌原纤维紧密结合和磷酸肌酸将能量从线粒体分流到肌原纤维的证据。
In hyperpermeable cardiac cells, in which the surface membrane has been made highly permeable to small molecules and ions, resting tension increases when the concentration of ATP falls below 200 microM. Peak resting tension occurs in 10 microM ATP and equals 60% of maximum Ca-activated force in 5 mM ATP. The mitochondria in hyperpermeable cells can maintain an ATP concentration above 200 microM if supplied with O2, substrate, ADP, and inorganic phosphate (Pi). Removal of ATP from the bathing solution does not increase resting tension as long as creatine phosphate is present. However O2, substrate, and Pi cannot lower resting tension in the absence of ATP and creatine phosphate. These results are interpreted as evidence for adenine nucleotide tightly bound to the myofibrils and a creatine phosphate shunt of energy from the mitochondria to the myofibrils.