Identification and developmental expression of new biomineralization proteins in the sea urchin Strongylocentrotus purpuratus
Identification and developmental expression of new biomineralization proteins in the sea urchin Strongylocentrotus purpuratus
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DOI：
10.1007/s00427-002-0261-0
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发表时间：
2002-10-01
影响因子：
2.4
通讯作者：
Ettensohn, CA
中科院分区：
文献类型：
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作者：
Illies, MR;Peeler, MT;Ettensohn, CA
海胆幼虫的内骨骼是由初级间充质细胞（PMC）分泌的钙质杆状物构成的网状结构。在这项研究中，我们确定了7个新的生物矿化相关蛋白，通过分析一个大型数据库的基因产物表达的PMC。这些蛋白质包括三种新的骨针基质蛋白（SpSM 29、SpSM 32和SpC-lectin）、两种与PMC特异性细胞表面糖蛋白MSP 130相关的蛋白质（MSP 130-related-1和-2）以及两种新蛋白质（SpP 16和SpP 19）。编码这些蛋白质的基因由大微粒体-PMC谱系的细胞特异性表达，并且在PMC侵入之前在囊胚阶段开始合子激活。几种mRNA在PMC合胞体内显示出与骨骼杆生长模式相关的受调节的表达模式。这项工作确定了新的蛋白质，可能会调节这个易处理的模型系统中的生物矿化过程。
The endoskeleton of the sea urchin larva is a network of calcareous rods secreted by primary mesenchyme cells (PMCs). In this study, we identified seven new biomineralization-related proteins through an analysis of a large database of gene products expressed by PMCs. The proteins include three new spicule matrix proteins (SpSM29, SpSM32, and SpC-lectin), two proteins related to the PMC-specific cell surface glycoprotein MSP130 (MSP130-related-1 and -2), and two novel proteins (SpP16 and SpP19). The genes encoding these proteins are expressed specifically by cells of the large micromere-PMC lineage and are activated zygotically beginning at the blastula stage, prior to PMC ingression. Several of the mRNAs show regulated patterns of expression within the PMC syncytium that correlate with the pattern of skeletal rod growth. This work identifies new proteins that may regulate the process of biomineralization in this tractable model system.