In Situ Determination of Colloidal Gold Concentrations with UV-Vis Spectroscopy: Limitations and Perspectives
In Situ Determination of Colloidal Gold Concentrations with UV-Vis Spectroscopy: Limitations and Perspectives
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DOI：
10.1021/ac502053s
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发表时间：
2014-11-18
影响因子：
7.4
通讯作者：
Polte, Joerg
中科院分区：
文献类型：
--
作者：
Hendel, Thomas;Wuithschick, Maria;Polte, Joerg
本文研究了胶体金纳米颗粒在400 nm处的紫外-可见吸收，并验证了其作为胶体金溶液中Au（0）浓度测定方法的可行性。该方法被证明是有效的，取决于调查系统的限制。测定Au（0）浓度的不确定度可达30%。这种偏差是颗粒尺寸、表面改性或氧化状态等参数综合影响的结果。然而，量化这些参数的影响使得能够对特定系统进行精确得多的Au（0）测定。作为一个例子，监测公知的Turkevich方法的还原过程，并测定Au（0）浓度，偏差小于5%。因此，证明了一种简单、快速、容易且廉价的用于Au（0）测定的原位方法，该方法在存在其他金物质例如Au（III）的情况下具有前所未有的准确度。
This paper studies the UV-vis absorbance of colloidal gold nanopartides at 400 nm and validates it as a method to determine Au(0) concentrations in colloidal gold solutions. The method is shown to be valid with restrictions depending on the investigated system. The uncertainty of the determined Au(0) concentration can be up to 30%. This deviation is the result of the combined influence of parameters such as particle size, surface modification, or oxidation state. However, quantifying the influence of these parameters enables a much more precise Au(0) determination for specific systems. As an example, the reduction process of the well-known Turkevich method was monitored and the Au(0) concentration was determined with a deviation of less than 5%. Hence, a simple, fast, easy, and cheap in situ method for Au(0) determination is demonstrated that has in the presence of other gold species such as Au(III) an unprecedented accuracy.