Arrays of horizontally-oriented mini-reservoirs generate steady microfluidic flows for continuous perfusion cell culture and gradient generation
Arrays of horizontally-oriented mini-reservoirs generate steady microfluidic flows for continuous perfusion cell culture and gradient generation
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DOI：
10.1039/b407623k
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发表时间：
2004-01-01
期刊：
影响因子：
4.2
通讯作者：
Takayama, S
中科院分区：
文献类型：
--
作者：
Zhu, XY;Chu, LY;Takayama, S
本文描述了使用水平取向的储液器阵列在长时间（数小时至数天）内以恒定流速通过微通道输送液体。水平取向保持跨微流体通道的恒定液压压降，即使在储存器内的液体体积随时间变化时。对于一个给定的渠道水库系统，流速的大小线性地依赖于水库之间的高度差。结构简单，操作机构简单，具有通用性。使用一入口一出口系统连续递送用于灌注细胞培养的培养基，并且使用经由微通道耦合到出口储器的入口储器阵列来驱动多个层流的流动。平行泵送方案方便地产生各种平滑和阶梯浓度梯度，并允许秋水仙碱对成肌细胞的影响进行评估。由于储存器阵列被配置为与设计用于96孔板处理的商业化多通道移液器兼容，因此设想这种简单的泵送方案广泛地用于中到高通量微流体灌注细胞培养测定、细胞迁移测定、多层流药物测试和需要多个微流体流的任何其他应用。
This paper describes the use of arrays of horizontally-oriented reservoirs to deliver liquids through microchannels at a constant flow rate over extended periods of time (hours to days). The horizontal orientation maintains a constant hydraulic pressure drop across microfluidic channels even as the volumes of liquids within the reservoirs change over time. For a given channel-reservoir system, the magnitude of the flow velocity depends linearly on the height difference between reservoirs. The simple structure and operation mechanism make this pumping system versatile. A one-inlet-one-outlet system was used to continuously deliver media for perfusion cell culture, and an array of inlet reservoirs coupled to an outlet reservoir via microchannels was used to drive flows of multiple laminar streams. The parallel pumping scheme conveniently generated various smooth and step concentration gradients, and allowed evaluation of the effect of colchicine on myoblasts. Since the reservoir arrays are configured to be compatible with commercialized multichannel pipettors designed for 96 well plate handling, this simple pumping scheme is envisioned to be broadly useful for medium to high throughput microfluidic perfusion cell culture assays, cell migration assays, multiple laminar flow drug tests, and any other applications needing multiple microfluidic streams.