The Two-Component System DcuS-DcuR Is Involved in Virulence and Stress Tolerance in the Poplar Canker Bacterium Lonsdalea populi
The Two-Component System DcuS-DcuR Is Involved in Virulence and Stress Tolerance in the Poplar Canker Bacterium Lonsdalea populi
复制标题
双组分系统 DcuS-DcuR 参与杨树溃疡病菌 Lonsdalea populi 的毒力和胁迫耐受性
DOI：
10.1094/phyto-03-20-0094-r
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发表时间：
2020
期刊：
影响因子：
3.2
通讯作者：
Li Aining
中科院分区：
文献类型：
--
作者：
Zheng Zeyang;Deng Chaoying;He Wei;Qian Wei;Li Aining
革兰氏阴性细菌 Lonsdalea populica 引起新出现的杨树（Populus×euramericana）溃疡病，导致中国和欧洲的杨树生产遭受严重损失。双组分信号转导系统在植物病原细菌毒力和胁迫反应的调节中发挥重要作用。我们在L中鉴定了一个二元对（Lqp2625-Lqp2624）。 populi，与大肠杆菌的DcuS-DcuR高度同源。缺乏DcuSorDcuR的突变体表现出正常生长，但它们对杨树枝的毒力受损。无法产生鞭毛表明 DcuS 和 DcuRa 参与生物膜形成和游泳运动。此外，DcuSorDcuR的缺失通过下调与过氧化氢酶和多药外排泵相关的基因导致对氧化应激和氯霉素的敏感性增加，这表明这两种成分对有助于细胞对氧化和抗生素应激的适应。我们确定了对毒力和应激反应重要的关键域和假定的磷酸化位点。我们的研究结果揭示了 DcuS-DcuR 在 L 毒力和应激反应中的功能。越来越多的证据表明，双组分系统在细菌的感染过程和应激适应过程中至关重要。
The gram-negative bacteriumLonsdalea populicauses an emerging poplar (Populus×euramericana) canker resulting in severe losses to poplar production in China and Europe. Two-component signal transduction systems play important roles in the regulation of virulence and stress responses in phytopathogenic bacteria. We identified a two-component pair (Lqp2625-Lqp2624) inL. populi, highly homologous to DcuS-DcuR ofEscherichia coli. Mutants lackingDcuSorDcuRdisplayed normal growth while their virulence on poplar twigs was impaired. An inability to produce flagella indicated thatDcuSandDcuRare involved in biofilm formation and swimming motility. Moreover, the loss ofDcuSorDcuRled to increased sensitivity to oxidative stress and chloramphenicol through downregulation of genes associated with catalases and the multidrug efflux pump, suggesting that the two-component pair contributes to cellular adaptation to oxidative and antibiotic stresses. We identified key domains and putative phosphorylation sites important for virulence and stress responses. Our findings reveal the functions of DcuS-DcuR in virulence and stress responses inL. populiand provide increasing evidence that two-component systems are crucial during the infection process and stress adaptation in bacteria.