EVOLUTION OF INFLUENZA-A AND INFLUENZA-B VIRUSES - CONSERVATION OF STRUCTURAL FEATURES IN THE HEMAGGLUTININ GENES
EVOLUTION OF INFLUENZA-A AND INFLUENZA-B VIRUSES - CONSERVATION OF STRUCTURAL FEATURES IN THE HEMAGGLUTININ GENES
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DOI：
10.1073/pnas.79.15.4800
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
PALESE, P
中科院分区：
文献类型：
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作者：
KRYSTAL, M;ELLIOTT, RM;PALESE, P
用从B型流感病毒(B/Lee/40)的RNA片段克隆的c[互补]DNA，获得了该病毒血凝素(HA)基因的全序列。该基因全长1882个核苷酸，可编码584个氨基酸的蛋白质前体。A型病毒HA共有的结构特征在B型病毒HA中也是保守的。它们包括HA2亚基的疏水信号肽、疏水、NH2和COOH末端，以及涉及精氨酸残基的HA1/HA2裂解位点。BHA基因及其推导的氨基酸序列与甲型流感病毒(A/PR/8/34)的序列进行了比较。当这两个基因比对时，发现HA1亚基中24%的氨基酸和HA2亚基中39%的氨基酸是保守的。B型病毒和A型病毒HA之间的这种相关性程度(型间比较)与某些A型病毒HA之间观察到的同源性(型内比较)相似。因此，A型流感病毒和B型流感病毒之间存在密切的进化关系。
The complete nucleotide sequence of the hemagglutinin (HA) gene of a type B influenza virus (B/Lee/40) was obtained by using cloned c[complementary]DNA derived from the RNA segment. The gene is 1882 nucleotides long and can code for a protein precursor of 584 aminio acids. Structural features common to type A virus HA are also conserved in the B virus HA. These include a hydrophobic signal peptide, hydrophobic, NH2 and COOH termini of the HA2 subunit and a HA1/HA2 cleavage site involving an arginine residue. The sequence of the B HA gene and its deduced amino acid sequence were compared to those of a type A influenza virus (A/PR/8/34). When these 2 genes were aligned, it was found that 24% of the amino acids in the HA1 subunits and 39% of the amino acids in the HA2 subunits are conserved. This degree of relatedness between type B virus and type A virus HA (intertypic comparison) is simlar to the homologies observed among certain type A virus HA (intratypic comparison). A close evolutionary relationship is therefore suggested between the HA of type A and type B influenza viruses.