Differential proteomics analysis of Frankliniella occidentalis immune response after infection with Tomato spotted wilt virus (Tospovirus)
Differential proteomics analysis of Frankliniella occidentalis immune response after infection with Tomato spotted wilt virus (Tospovirus)
复制标题
DOI：
10.1016/j.dci.2016.10.013
复制
发表时间：
2017-02-01
影响因子：
2.9
通讯作者：
Poehling, Hans-Michael
中科院分区：
文献类型：
--
作者：
Ogada, Pamella Akoth;Kiirika, Leonard Muriithi;Poehling, Hans-Michael
番茄斑萎病毒（Tomato spotted wilt virus，TSWV）主要由西花蓟马（Frankliniella ococcuentalis Pergande）介导，它能激活介导体的免疫应答。然而，改变的免疫应答的分子背景尚不清楚。因此，使用蛋白质组学方法，我们研究了在F。在暴露于TSWV 24 h后，采用双向等电聚焦/十二烷基硫酸钠聚丙烯酰胺凝胶电泳（2D-IEF/SDS/PAGE）结合质谱（MS）鉴定病毒感染后差异表达的蛋白质。与未暴露的（27%）相比，暴露于TSWV的Eococcientalis（73%）中大量表达了大量蛋白质，其中大多数功能与先天免疫系统相关，例如：信号传导，应激反应，防御反应，翻译，细胞脂质和核苷酸代谢。关键蛋白质包括：70 kDa热休克蛋白、泛素和Dermcidin等，表明载体的先天免疫系统对病毒感染的应答模式。(C)2016爱思唯尔有限公司版权所有
Tomato spotted wilt virus (TSWV) is mainly vectored by Frankliniella occidentalis Pergande, and it potentially activates the vector's immune response. However, molecular background of the altered immune response is not clearly understood. Therefore, using a proteomic approach, we investigated the immune pathways that are activated in F. occidentalis larvae after 24 h exposure to TSWV. Two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D-IEF/SDS/PAGE) combined with mass spectrometry (MS), were used to identify proteins that were differentially expressed upon viral infection. High numbers of proteins were abundantly expressed in E occidentalis exposed to TSWV (73%) compared to the non-exposed (27%), with the majority functionally linked to the innate immune system such as: signaling, stress response, defense response, translation, cellular lipids and nucleotide metabolism. Key proteins included: 70 kDa heat shock proteins, Ubiquitin and Dermcidin, among others, indicative of a responsive pattern of the vector's innate immune system to viral infection. (C) 2016 Elsevier Ltd. All rights reserved.