Microfilaments: dynamic arrays in higher plant cells
Microfilaments: dynamic arrays in higher plant cells
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微丝：高等植物细胞中的动态阵列
DOI：
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发表时间：
1987
影响因子：
7.8
通讯作者：
C. Weerdenburg
中科院分区：
文献类型：
--
作者：
R. Seagull;M. Falconer;C. Weerdenburg
通过使用荧光标记的鬼笔环肽，我们在光学显微镜水平上检查了植物细胞周期和分化过程中肌动蛋白样微丝（mfs）的三维分布和重组。在间期，mfs 被组织成三个不同但相互关联的阵列：靠近质膜的精细外围网络；皮层下区域有大的轴向电缆；延伸至跨液泡链的 mfs 核“篮子”。所有这些阵列从外周网络开始，在有丝分裂开始时消失，并在胞质分裂后从核篮开始重新出现。在有丝分裂和细胞因子事件期间，mfs 与纺锤体和成膜体相关。纺锤体中的肌动蛋白染色位于染色体和纺锤体极之间，并以功能特异性方式发生变化。核区似乎是 mf 组织和/或启动的中心。在从快速细胞分裂到细胞伸长的分化过程中，mf 阵列从轴向转变为横向，从而平行于微管。这种方向的变化反映了细胞质流方向的转变。这些观察结果首次表明，肌动蛋白样 mfs 在植物细胞中形成复杂且动态的阵列，这可能涉及许多尚未描述的细胞功能。
By using fluorescently labeled phalloidin we have examined, at the light microscope level, the three-dimensional distribution and reorganization of actin-like microfilaments (mfs) during plant cell cycle and differentiation. At interphase, mfs are organized into three distinct yet interconnected arrays: fine peripheral networks close to the plasma membrane; large axially oriented cables in the subcortical region; a nuclear "basket" of mfs extending into the transvacuolar strands. All these arrays, beginning with the peripheral network, disappear at the onset of mitosis and reappear, beginning with the nuclear basket, after cytokinesis. During mitotic and cytokinetic events, mfs are associated with the spindle and phragmoplast. Actin staining in the spindle is localized between the chromosomes and the spindle poles and changes in a functionally specific manner. The nuclear region appears to be the center for mf organization and/or initiation. During differentiation from rapid cell division to cell elongation, mf arrays switch from an axial to a transverse orientation, thus paralleling the microtubules. This change in orientation reflects a shift in the direction of cytoplasmic streaming. These observations show for the first time that actin-like mfs form intricate and dynamic arrays in plant cells which may be involved in many as yet undescribed cell functions.
体外早期内皮再生过程中肌动蛋白细胞骨架的组织。
影响因子：
4
作者：
Gabbiani,G;Gabbiani,F;Heimark,RL;Schwartz,SM
通讯作者：
Schwartz,SM