Regulation of Light Harvesting inChlamydomonas reinhardtiiTwo Protein Phosphatases Are Involved in State Transitions1[OPEN]
Regulation of Light Harvesting inChlamydomonas reinhardtiiTwo Protein Phosphatases Are Involved in State Transitions1[OPEN]
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DOI：
10.1104/pp.20.00384
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发表时间：
2020-08-01
期刊：
影响因子：
7.4
通讯作者：
Goldschmidt-Clermont，Michel
中科院分区：
文献类型：
--
作者：
Cariti，Federica;Chazaux，Marie;Goldschmidt-Clermont，Michel
蛋白质磷酸化在光合电子传递的短期调节中起重要作用，在状态转换过程中，莱茵衣藻的激酶STATE TRANSITION 7（STT 7）磷酸化捕光天线复合体II（LHCII）的组分。这种可逆的磷酸化控制着一部分LHCII向PSI或PSII的动态分配，这取决于光照条件和代谢需求，但对抗磷酸酶在C中仍然未知。莱因哈德氏菌在这里，我们分析了C中的状态转换。两种磷酸酶，蛋白质磷酸酶1和光系统II磷酸酶的再硬化突变体，它们与拮抗拟南芥中状态转换激酶（STN 7和STN 8）的蛋白质同源。从状态2到状态1的转变在pph 1中被延迟，并且令人惊讶地在pbcp中也被延迟。然而，这两个突变体最终都回到了状态1。相比之下，双muph 1; pbcp出现强烈锁定在状态2。LHCII三聚体和单体亚基的复杂磷酸化模式在磷酸酶突变体中受到影响。他们的分析表明，这两种磷酸酶具有不同但重叠的蛋白质靶点。C.类囊体蛋白脱磷酸化和更复杂的天线磷酸化模式的双重控制。reinhardtiicombranes到拟南芥的讨论的背景下，更强的振幅的状态转换和更多样化的LHCII亚型的拟南芥。
Protein phosphorylation plays important roles in short-term regulation of photosynthetic electron transfer, and during state transitions, the kinase STATE TRANSITION7 (STT7) ofChlamydomonas reinhardtiiphosphorylates components of light-harvesting antenna complex II (LHCII). This reversible phosphorylation governs the dynamic allocation of a part of LHCII to PSI or PSII, depending on light conditions and metabolic demands, but counteracting phosphatase(s) remain unknown inC. reinhardtii. Here we analyzed state transitions inC. reinhardtiimutants of two phosphatases, PROTEIN PHOSPHATASE1 and PHOTOSYSTEM II PHOSPHATASE, which are homologous to proteins that antagonize the state transition kinases (STN7 and STN8) in Arabidopsis (Arabidopsis thaliana). The transition from state 2 to state 1 was retarded inpph1, and surprisingly also inpbcp. However, both mutants eventually returned to state 1. In contrast, the double mutantpph1;pbcpappeared strongly locked in state 2. The complex phosphorylation patterns of the LHCII trimers and of the monomeric subunits were affected in the phosphatase mutants. Their analysis indicated that the two phosphatases have different yet overlapping sets of protein targets. The dual control of thylakoid protein dephosphorylation and the more complex antenna phosphorylation patterns inC. reinhardtiicompared to Arabidopsis are discussed in the context of the stronger amplitude of state transitions and the more diverse LHCII isoforms in the alga.