Molecular typing of the etiologic agent of human granulocytic ehrlichiosis.
Molecular typing of the etiologic agent of human granulocytic ehrlichiosis.
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人粒细胞埃立克体病病原体的分子分型。
DOI：
10.1128/jcm.39.9.3398-3401.2001
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发表时间：
2001
影响因子：
9.4
通讯作者：
Johnson,RC
中科院分区：
文献类型：
--
作者：
Carter,SE;Ravyn,MD;Xu,Y;Johnson,RC
人粒细胞埃利希体病（aoHGE）因子的p44基因编码一个44 kda的主要外表面蛋白。建立了一种基于p44基因PCR扩增的aoHGE分型技术，然后用hindiii、EcoRV和aspi进行多限制性内切酶切，生成限制性内切片段长度多态性模式。从美国地理位置分散的地点收集了24份aoHGE样本，包括人类、马、犬、小型哺乳动物和蜱的分离株。鉴定出6种粒细胞埃里希体病（GE）类型。GE分型方法操作相对简单，具有可重复性，并且能够在美国的各种粒细胞埃希体分离株之间进行区分。这些特点表明，这种GE分型方法可能是一种重要的流行病学和流行病学工具。
Thep44gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of thep44gene followed by a multiple restriction digest withHindIII,EcoRV, andAspI to generate restriction fragment length polymorphism patterns. Twenty-four samples of the aoHGE were collected from geographically dispersed sites in the United States and included isolates from humans, equines, canines, small mammals, and ticks. Six granulocytic ehrlichiosis (GE) types were identified. The GE typing method is relatively simple to perform, is reproducible, and is able to differentiate among the various isolates of granulocytic ehrlichiae in the United States. These characteristics suggest that this GE typing method may be an important epizootiological and epidemiological tool.