The effect of storage environment on the longevity of conidia of Beauveria bassiana
The effect of storage environment on the longevity of conidia of Beauveria bassiana
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DOI：
10.1017/s0953756201004026
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Moore, D
中科院分区：
文献类型：
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作者：
Hong, TD;Gunn, J;Moore, D
通过估算生活力方程的参数，研究了风干贮藏环境对7个不同时间和地点产生的球孢白僵菌（Beauveria bassiana）分生孢子寿命的影响。将分生孢子密封储存在6至11个含水量在2.3和32.0%之间的环境中，在1至5个恒定温度（50 +/-0.5 ℃）下（10、20、30、40和50 +/-0.5 ℃）储存不同的时间，最长372天，然后测试存活力。水分含量（C-W）和温度（C-H和C-Q）对分生孢子寿命的相对影响在所有菌株中表现相似（P > 0.25），共同值分别为C-W = 3.05（SE = 0.07）、C-H = 0.0293（SE = 0.0078）和C-Q = 0.00081（SE = 0.00011）。在50度和40度下，分离物198- 1140 ss的分生孢子含水量和寿命之间的负对数关系的低含水量限度估计值分别为4.6%和5.0%，分离物197-1111的含水量分别为5.2%和5.1%。不同菌株间的绝对寿命（K-E）差异显著（P < 0.005），即使同一菌株内，分生孢子在不同位置产生时也是如此。在7个分离株的8个样本中，确定了两个队列的K-E（P < 0.005）：在阿斯科特产生的3个分离株的分生孢子的K-E的共同估计值为6.696（SE = 0.170），而在内罗毕或卡罗莱纳产生的分生孢子的K-E估计值较低（6.203，SE = 0.029）。K-E的这种差异意味着，对于任何给定环境中的任何给定存活期，在阿斯科特中产生的分生孢子提供的寿命约为其他样品的三倍。
The effect of air-dry storage environment on the longevity of conidia from seven isolates of Beauveria bassiana produced at different times and locations was determined by estimating the parameters of a viability equation. Conidia were stored hermetically at six to II moisture contents between 2.3 and 32.0% with one (50 +/- 0.5 degreesC) to five constant temperatures (10, 20, 30, 40 and 50 +/- 0.5 degrees) for various periods up to 372 d and then tested for viability. All isolates behaved similarly (P > 0.25) in terms of the relative effect of moisture content (C-W) and temperature (C-H and C-Q) on conidial longevity; common values were C-W = 3.05 (SE = 0.07), C-H = 0.0293 (SE = 0.0078), and C-Q = 0.00081 (SE = 0.00011). Estimates of the low-moisture-content limit to the negative logarithmic relation between conidial moisture content and longevity were 4.6 and 5.0% at 50 degrees and 40 degrees, respectively, for isolate 198-1140ss, and 5.2 and 5.1% moisture content, respectively, for isolate 197-1111. Absolute longevity (K-E) varied considerably (P < 0.005) among isolates, even within an isolate when conidia were produced at different locations. Among the eight samples of seven isolates, two cohorts were identified with respect to K-E (P < 0.005): conidia of three isolates which were produced at Ascot had a common estimate of K-E of 6.696 (SE = 0.170), whereas those produced at Nairobi or Carolina provided a lower estimate (6.203, SE = 0.029). This difference in K-E means that for any given viability period in any given environment, the conidia produced in Ascot provided about three times the longevity of the other samples.