Effects of perinatal exposure to a polybrominated diphenyl ether (PBDE 99) on mouse neurobehavioural development
Effects of perinatal exposure to a polybrominated diphenyl ether (PBDE 99) on mouse neurobehavioural development
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DOI：
10.1016/s0161-813x(02)00078-5
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发表时间：
2002-09-01
期刊：
影响因子：
3.4
通讯作者：
Costa, LG
中科院分区：
文献类型：
--
作者：
Branchi, I;Alleva, E;Costa, LG
多溴联苯醚（PBDEs）是一类广泛使用的阻燃剂，正如对哨兵动物物种和人类的几项研究所表明的那样，它在环境中广泛扩散。特别令人关切的是，据报告，母乳中多溴二苯醚的含量很高，因为几乎没有关于其对发育中的生物体的潜在影响的信息。我们研究了围产期多溴二苯醚暴露对小鼠神经行为发育的影响。从妊娠第6天（GD）至出生后第21天（PND），每天通过管饲法向CD-1瑞士雌性动物给予2，2 '，4，4'，5-五溴二苯醚（PBDE 99;每天0.6、6和30毫克/千克）。多氯联苯混合物Aroclor 1254（A1254; 6 mg/kg/天）按照相同的时间表给药，并作为阳性对照。中等剂量的PBDE 99对幼仔的生存能力有影响。感觉运动发育分析（PND 2-20）显示，PBDE 99高剂量组的攀爬反应出现延迟。PND 11时，归巢试验显示，给药动物（尤其是A1254组）的活动趋势高于对照组。这种活性水平的变化强烈增加PND 34和60在一个开放的领域竞技场。在PND 60，治疗的小鼠也显示出改变的趋触性，在竞技场的中心比对照花费更多的时间。在成年期，A1254处理过的小鼠仍然活动过度，而PBDE 99处理过的小鼠往往活动不足。这些研究结果表明，围产期接触多溴二苯醚99会产生若干行为改变，其影响并不总是与A1254相似。新生儿接触多溴二苯醚的可能性值得进一步研究，以证实其发育神经毒性。(C)2002年爱思唯尔科技有限公司All rights reserved.
Polybrominated diphenyl ethers (PBDEs), a class of widely used flame retardants, are extensively diffused in the environment as shown by several studies on sentinel animal species, as well as humans. Of particular concern are the reported high levels of PBDEs in human milk, as almost no information is available on their potential effects on developing organisms. We investigated the effects of perinatal PBDE exposure on mouse neurobehavioural development. 2,2',4,4',5-pentabromodiphenylether (PBDE 99; 0.6, 6 and 30 mg/kg per day) was administered daily to CD-1 Swiss females by gavage from gestational day (GD) 6 to postnatal day (PND) 21. Aroclor 1254 (A1254; 6 mg/kg per day), a PCB mixture, was administered following the same schedule and served as a positive control. The PBDE 99 medium dose had an effect on litter viability. Sensori-motor development analysis (PNDs 2-20) revealed a delayed appearance of climbing response in the PBDE 99 high-dose group. On PND 11, the homing test revealed a trend for treated animals, particularly the A1254 group, to be more active than controls. This activity level alteration was strongly increased on PNDs 34 and 60 in an open-field arena. On PND 60, treated mice showed also an altered thigmotaxis, spending more time in the centre of the arena than controls. At adulthood, A1254 treated mice were still hyperactive, whereas the PBDE 99 groups tended to be hypoactive. These findings showed that perinatal exposure to PBDE 99 produces several behavioural alterations and that its effects are not always similar to those of A1254. The possibility of exposure of neonates to PBDEs warrants further studies to characterise their developmental neurotoxicity. (C) 2002 Elsevier Science Inc. All rights reserved.