Genotypic Detection of qnrA and qnrC Genes in Citrobacter koseri Isolated from Patients with Urinary Tract Infection.
Genotypic Detection of qnrA and qnrC Genes in Citrobacter koseri Isolated from Patients with Urinary Tract Infection.
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DOI：
10.22092/ari.2022.357122.1979
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发表时间：
2022-04-01
影响因子：
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通讯作者：
Sabah Bustani, G
中科院分区：
文献类型：
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作者：
Sadeq Al-Ethari, A;Hayder Hasan, T;Sabah Bustani, G
泌尿道感染（UTI）是一种流行的感染，影响所有年龄段的人。细菌性病原体是尿路感染最常见的原因。肺炎克雷伯氏菌、奇异变形杆菌、铜绿假单胞菌和其他葡萄球菌、弗氏柠檬酸杆菌和科氏柠檬酸杆菌（C. koseri）感染人数较少。这些病原体从结肠生态位进入泌尿道，进入菌群失调。随机收集249例疑似尿路感染的门诊患者的尿液样本。生殖器清洁后，在无菌肠中采集10 mL尿液样本。然后，将样本以2，000 rpm离心5 min，并将残留物与脑烧瓶的肉汤灌注液在37°C下有氧孵育24 h，然后用无菌环施加到血琼脂平板和MacConkey琼脂（OxoidTM）上。249份尿样中革兰阴性菌176株（70.7%），革兰阳性菌51株（20.5%）。然而，结果表明，有22个（8.8%）尿样无生长。此外，本研究还发现了8种不同的抗菌药物对C. koseri。在本研究中，C.用八种不同的抗微生物剂处理koseri。对头孢噻肟、头孢曲松、环丙沙星、左氧氟沙星的耐药率较高的有6株（85.71%）。结果表明，6株和5株分离菌对头孢他啶和左氧氟沙星的耐药率分别为85.71%和71.42%。庆大霉素耐药率为57.14%，丁胺卡那霉素耐药率为28.57%。分离菌对亚胺培南敏感率高。qnrA基因阳性6株（85.71%）。然而，记录的数据表明没有携带qnrC基因的分离株。在所有致病菌中，C. Koseri是本次研究中UTI的最低致病菌，对大多数抗菌药物均高度耐药，但亚胺培南是一种良好的抗生素，敏感性为100%。
The urinary tract infection (UTI) is a prevalent infection that affects people of all ages. Bacterial agents are the most common causes of UTIs. Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and other staphylococcal species, Citrobacter freundii and Citrobacter koseri (C. koseri) account for a smaller number of infections. These pathogens are transported into the urinary tract from the colonic biotope into dysbacteriosis. Urine samples were randomly collected from 249 outpatients who were suspected of having UTIs. After genital cleaning, 10 mL of urine specimens were collected in a sterilized bowel. Then, the specimens were centrifuged at 2,000 rpm for 5 min and the residue was aerobically incubated with the broth infusion of brain flasks at 37°C for 24 h and then applied with a sterile ring onto blood agar plates and MacConkey agar (OxoidTM). Out of 249 urine samples, the results proved that there were 176 (70.7%) and 51(20.5%) gram-negative and gram-positive bacteria isolates, respectively. However, the results demonstrated that there were 22 (8.8%) urine samples with no growth. In addition, the results showed that eight various antimicrobials are used to treat C. koseri. In the current study, C. koseri was treated with eight different antimicrobial agents. The antimicrobial resistance rate for 7 isolates against Cefotaxime, Ceftriaxone, Ciprofloxacin, and Levofloxacin was high for 6 (85.71%) isolates. The results indicated that 6 and 5 isolates had 85.71% and 71.42% antimicrobial resistance against Ceftazidime and Levofloxacin, respectively. Whereas Gentamicin showed a moderate rate of resistance (4 isolates, 57.14%), and Amikacin resistance was found in 5 isolates, accounting for 28.57%. The bacterial isolates had a high susceptibility rate to Imipenem. The qnrA gene was found in 6 (85.71%) isolates. However, the recorded data demonstrated that there is no isolate carrying the qnrC gene. Among all pathogenic bacteria, C. koseri was the lowest causative agent of UTI in this study and was highly resistant to most antimicrobials except Imipenem, which was a good antibiotic with 100% sensitivity.