A mutant of Escherichia coli auxotrophic for organic phosphates: Evidence for two defects in inorganic phosphate transport
A mutant of Escherichia coli auxotrophic for organic phosphates: Evidence for two defects in inorganic phosphate transport
复制标题
有机磷酸盐营养缺陷型大肠杆菌突变体：无机磷酸盐运输中两个缺陷的证据
DOI：
10.1007/bf00269422
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
J. Cronan
中科院分区：
文献类型：
--
作者：
G. Sprague;R. Bell;J. Cronan
摘要一种无机磷转运突变体已被分离为sn-甘油-3-磷酸营养缺陷型，并对其进行了遗传学特征分析。两个病变是运输缺陷的原因。一个病变，PST，位于大肠杆菌遗传图谱的第74分钟，而另一个病变，PIT，位于68分钟。所有被检测的“K10”菌株都带有坑斑。有证据表明，磷酸甘油和己糖磷酸转运系统。作图数据表明，Mala和Xyl之间的遗传距离比现在允许的距离要大。
SummaryAn inorganic phosphate transport mutant has been isolated as a sn-glycerol-3-phosphate auxotroph and characterized genetically. Two lesions are responsible for the transport defect. One lesion, pst, is located at minute 74 of the E. coli genetic map while the other lesion, pit, is located at minute 68. All “K10” strains that were examined carry the pit lesion. Evidence is presented that the glycerol phosphate and hexose phosphate transport systems. The mapping data indicate that the genetic distance between malA and xyl is greater than that now allowed.