Selectide Technology: Substrate Motif Identification for Post-translational Modifications
Selectide Technology: Substrate Motif Identification for Post-translational Modifications
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Selectide 技术：翻译后修饰的底物基序识别
DOI：
10.1006/meth.1994.1041
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发表时间：
1994
期刊：
影响因子：
4.8
通讯作者：
Jinzi J. Wu
中科院分区：
文献类型：
--
作者：
K. Lam;Jinzi J. Wu
摘要基于“一珠一肽概念”的随机合成肽库筛选方法（Selectide Process）可作为一种通用的肽底物模体翻译后修饰鉴定方法。我们将这种方法应用于cAMP依赖性蛋白激酶系统，并能够快速识别出与其天然底物完全匹配的肽基序（RRXS）。
Abstract The random synthetic peptide library screening method based on the "one-bead one-peptide concept" (Selectide Process) can be used as a general method for the identification of peptide substrate motif for post-translational modifications. We applied this method to the cAMP-dependent protein kinase system and were able to rapidly identify a peptide motif (RRXS) that exactly matched that described for its natural substrates.