Isolation and characterization of a single-stranded RNA virus infecting the marine planktonic diatom Chaetoceros tenuissimus Meunier

Isolation and characterization of a single-stranded RNA virus infecting the marine planktonic diatom Chaetoceros tenuissimus Meunier
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DOI:
10.1128/aem.00509-08
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发表时间:
2008-07-01
影响因子:
4.4
通讯作者:
Nagasaki, Keizo
Nagasaki, Keizo
中科院分区:
生物学2区
文献类型:
--
作者:
Shirai, Yoko;Tomaru, Yuji;Nagasaki, Keizo

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硅藻是水生环境中生物群落和食物网的重要组成部分。在这里,我们报告的特点,单链RNA(ssRNA)病毒(CtenRNAV01)感染海洋硅藻Chaetoceros tenuissimus Meunier(硅藻)。CA。31 nm病毒颗粒为二十面体,无尾。CtenRNAV01形成占据感染宿主细胞质大部分的晶体阵列。通过生长实验,估计了裂解周期和爆发大小为:
Diatoms are important components of the biological community and food web in the aquatic environment. Here, we report the characteristics of a single-stranded RNA (ssRNA) virus (CtenRNAV01) that infects the marine diatom Chaetoceros tenuissimus Meunier (Bacillariophyceae). The ca. 31-nm virus particle is icosahedral and lacks a tail. CtenRNAV01 forms crystalline arrays occupying most of the infected host's cytoplasm. By growth experiments, the lytic cycle and the burst size were estimated to be