Monascus Azaphilone Pigment Biosynthesis Employs a Dedicated Fatty Acid Synthase for Short Chain Fatty Acyl Moieties
Monascus Azaphilone Pigment Biosynthesis Employs a Dedicated Fatty Acid Synthase for Short Chain Fatty Acyl Moieties
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DOI：
10.1007/s13765-014-4017-0
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发表时间：
2014-04-01
影响因子：
--
通讯作者：
Kwon, Hyung-Jin
中科院分区：
文献类型：
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作者：
Balakrishnan, Bijinu;Kim, Hyun-Ju;Kwon, Hyung-Jin
Monoclonal azaphilone pigments（MAzPs）的生物合成基因簇编码典型脂肪酸合成酶MpFAS 2。因此，提出MpFAS 2通过提供短链（C8和C10）脂肪酰基部分在MAzP生物合成中起作用。在紫红Monasterpurpureus中MpfasB2的靶向基因失活产生F9突变体，其产生白色菌丝，其在马铃薯葡萄糖琼脂上释放黄色。高效液相色谱分析表明，F9不能产生MAzP，而是积累了大量的发色团化合物。在F9中发现的主要化合物是红曲霉酮A，一种缺乏脂肪酰基部分的氢化氮杂菲酮。脂肪酸甲酯的气相色谱分析表明，WT和F9之间的细胞脂肪酰基（C16和C18）含量无显著差异。本研究表明，专用的脂肪酸合成酶是必需的，以装饰的azaphilone聚酮在MAzP的生物合成。
The biosynthetic gene cluster of Monascus azaphilone pigments (MAzPs) encodes a canonical fatty acid synthase, MpFAS2. It is thus proposed that MpFAS2 plays a role in MAzP biosynthesis by supplying short chain (C8 and C10) fatty acyl moieties. Targeted gene inactivation of MpfasB2 in Monascus purpureus generated an F9 mutant, which developed white hyphae that discharged a yellow color on potato dextrose agar. High-performance liquid chromatography analysis demonstrated that F9 was incapable of producing MAzP and accumulated a wide array of chromophoric compounds instead. The main compound found in F9 was monascusone A, a hydrogenated azaphilone lacking a fatty acyl moiety. Gas chromatography analysis of the fatty acid methyl esters indicated that there was no significant difference in the cellular fatty acyl (C16 and C18) contents between WT and F9. The present study demonstrates that the dedicated fatty acid synthase is required to decorate the azaphilone polyketides in MAzP biosynthesis.