NPFXD-mediated endocytosis is required for polarity and function of a yeast cell wall stress sensor.
NPFXD-mediated endocytosis is required for polarity and function of a yeast cell wall stress sensor.
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DOI：
10.1091/mbc.e06-08-0721
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发表时间：
2006-10
影响因子：
3.3
通讯作者：
H. Piao;Iara M P Machado;G. Payne
中科院分区：
文献类型：
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作者：
H. Piao;Iara M P Machado;G. Payne
肌动蛋白相关蛋白Sla 1 p通过其SHD 1结构域作为酵母中NPFX（1，2）D内吞靶向信号的接头。在这里，我们报告Wsc 1 p，细胞壁应力传感器，依赖于这个信号适配器对的内吞作用。Wsc 1 p中NPFDD的突变或缺乏SHD 1的Sla 1 p的表达阻断了Wsc 1 p的内化。通过活细胞成像，内吞缺陷Wsc 1 p不集中在网站的内吞。Wsc 1 p的极化分布的细胞生长的区域丢失的情况下的内吞作用。内体到高尔基体交通所必需的基因突变引起Wsc 1 p从细胞表面到内部隔室的重新分配，表明回收。Wsc 1 p内吞作用的抑制导致缺陷的极化沉积的细胞壁和增加的敏感性扰动的细胞壁合成。我们的结果表明，NPFX（1，2）D-Sla 1 p系统负责引导Wsc 1 p进入维持酵母细胞壁极性所需的内吞和回收途径。Wsc 1 p是酵母细胞外壁的一种传感器，其动态定位类似于某些细胞外基质感应整合素通过内吞再循环的极化分布。
The actin-associated protein Sla1p, through its SHD1 domain, acts as an adaptor for the NPFX(1,2)D endocytic targeting signal in yeast. Here we report that Wsc1p, a cell wall stress sensor, depends on this signal-adaptor pair for endocytosis. Mutation of NPFDD in Wsc1p or expression of Sla1p lacking SHD1 blocked Wsc1p internalization. By live cell imaging, endocytically defective Wsc1p was not concentrated at sites of endocytosis. Polarized distribution of Wsc1p to regions of cell growth was lost in the absence of endocytosis. Mutations in genes necessary for endosome to Golgi traffic caused redistribution of Wsc1p from the cell surface to internal compartments, indicative of recycling. Inhibition of Wsc1p endocytosis caused defects in polarized deposition of the cell wall and increased sensitivity to perturbation of cell wall synthesis. Our results reveal that the NPFX(1,2)D-Sla1p system is responsible for directing Wsc1p into an endocytosis and recycling pathway necessary to maintain yeast cell wall polarity. The dynamic localization of Wsc1p, a sensor of the extracellular wall in yeast, resembles polarized distribution of certain extracellular matrix-sensing integrins through endocytic recycling.