Morphology of VIP/nNOS-immunoreactive myenteric neurons in the human gut
Morphology of VIP/nNOS-immunoreactive myenteric neurons in the human gut
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DOI：
10.1007/s00418-005-0107-8
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发表时间：
2006-05-01
影响因子：
2.3
通讯作者：
Neuhuber, W
中科院分区：
文献类型：
--
作者：
Brehmer, A;Schrödl, F;Neuhuber, W
在这项研究中，我们的特点是人类肌间神经元共同免疫反应的神经元型一氧化氮合酶（nNOS）和血管活性肠肽（VIP）的形态和它们的比例相关的推定的整个肌间神经元的人口。对9例患者的9个全肠道（小肠和大肠样品）进行VIP、神经丝（NF）和nNOS三重染色。大多数神经元免疫反应的所有三个标志物显示放射状发射，部分分支树突与多刺的结束。这些神经元被称为棘神经元。在小肠标本中，它们的树突的棘状特征更为明显，与前面描述的脑啡肽能神经元明显不同。仅在十二指肠，一些神经元显示突出的主树突与多刺的侧支。在可从肌间神经丛的初级股内的起源神经节追踪到下一个神经节的轴突中（140个追踪神经元中的70个），94.3%经肛门和5.7%经口。很少有神经元反应的VIP和nNOS不能进行形态学分类，由于弱或缺乏NF-免疫反应。另外6只全标本进行VIP、nNOS和Hu蛋白（HU）三重染色。VIP/nNOS共反应神经元与HU反应神经元数量的比例在小肠标本中为5.8 - 11.5%，在大肠标本中为10.6 - 17.5%。我们的结论是，人肌间棘神经元的VIP和nNOS共同免疫反应代表抑制运动或下降的中间神经元。
In this study, we characterized human myenteric neurons co-immunoreactive for neuronal nitric oxide synthase (nNOS) and vasoactive intestinal peptide (VIP) by their morphology and their proportion as related to the putative entire myenteric neuronal population. Nine wholemounts (small and large intestinal samples) from nine patients were triple-stained for VIP, neurofilaments (NF) and nNOS. Most neurons immunoreactive for all three markers displayed radially emanating, partly branching dendrites with spiny endings. These neurons were called spiny neurons. The spiny character of their dendrites was more pronounced in the small intestinal specimens and differed markedly from enkephalinergic stubby neurons described earlier. Exclusively in the duodenum, some neurons displayed prominent main dendrites with spiny side branches. Of the axons which could be followed from the ganglion of origin within primary strands of the myenteric plexus beyond the next ganglion (70 out of 140 traced neurons), 94.3% run anally and 5.7% orally. Very few neurons reactive for both VIP and nNOS could not be morphologically classified due to weak or absent NF-immunoreactivity. Another six wholemounts were triple-stained for VIP, nNOS and Hu proteins (HU). The proportion of VIP/nNOS-coreactive neurons in relation to the number of HU-reactive neurons was between 5.8 and 11.5% in the small and between 10.6 and 17.5% in the large intestinal specimens. We conclude that human myenteric spiny neurons co-immunoreactive for VIP and nNOS represent either inhibitory motor or descending interneurons.