Long-term potentiation is associated with increased [3H]AMPA binding in rat hippocampus.
Long-term potentiation is associated with increased [3H]AMPA binding in rat hippocampus.
复制标题
长时程增强与大鼠海马中[3H]AMPA 结合的增加有关。
DOI：
10.1016/0006-8993(92)90767-4
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Thompson,RF
中科院分区：
文献类型：
--
作者：
Tocco,G;Maren,S;Shors,TJ;Baudry,M;Thompson,RF
长时程增强（LTP）的位置和性质的变化仍然是有争议的问题。在本研究中，我们测试了谷氨酸受体的α-氨基-3-羟基-5-甲基-4-异恶唑丙酸酯（AMPA）/使君子酸和N-甲基-d-天冬氨酸（NMDA）亚型结合特性的变化与LTP相关的可能性。在麻醉大鼠中，通过刺激穿通通路在体内诱发LTP。刺激后1小时处死动物。我们用[3 H]AMPA和[3 H]N-（1-（2-噻吩基）环己基）-3，4-哌啶（[3 H]TCP）分别标记AMPA/quisqualate和NMDA受体，在冰冻脑切片上进行定量配体结合放射自显影。在任何处理组中均未检测到[3 H]TCP结合的变化。然而，[3 H]AMPA结合的增加仅在表现出LTP的动物中观察到。这些增加是双侧的，存在于海马和皮质区的几个亚区。在强直刺激前给予NMDA受体拮抗剂氯胺酮可防止结合增加和LTP诱导。这些结果表明，AMPA/使君子酸受体的特性的变化是LTP的生化相关。
The location and nature of the changes underlying long-term potentiation (LTP) remain controversial issues. In this study, we tested the possibility that changes in binding properties of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)/quisqualate andN-methyl-d-aspartate (NMDA) subtype of glutamate receptors are associated with LTP. LTP was elcited in vivo by stimulation of the perforant pathway in anesthetized rats. One hour following stimulation the animals were sacrificed. We performed quantitative ligand binding autoradiography on frozen brain sections using [3H]AMPA and3H]N-(1-(2-thienyl)cyclohexyl)-3,4-piperidine ([3H]TCP) to label the AMPA/quisqualate and the NMDA receptors, respectively. No changes in [3H]TCP binding were detected in any of the treatment groups. However, increases in [3H]AMPA binding were observed only in animals that exhibited LTP. These increases were bilateral and present in several subfields of the hippocampus and cortical areas. Administration of the NMDA receptor antagonist, ketamine, prior to tetanic stimulation prevented both the increase in binding and the induction of LTP. These results suggest that changes in the characteristics of AMPA/quisqualate receptors are a biochemical correlate of LTP.