A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer's disease genes and Aβ
A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer's disease genes and Aβ
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DOI：
10.1073/pnas.101133498
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发表时间：
2001-05-22
影响因子：
11.1
通讯作者：
Nishimoto, I
中科院分区：
文献类型：
--
作者：
Hashimoto, Y;Niikura, T;Nishimoto, I
通过功能表达筛选，我们鉴定了一个基因，命名为Humanin (HN) cDNA，它编码一个短多肽，并消除由多种不同类型的家族性阿尔茨海默病基因和Aβ淀粉样蛋白引起的神经元细胞死亡，而对Q79或超氧化物歧化酶-1突变体引起的死亡没有影响。转染的HN cDNA被转录为相应的多肽，然后分泌到培养基中。这种拯救作用显然取决于 HN 的一级结构。这种多肽可以作为开发针对阿尔茨海默病神经保护的新疗法的分子线索。
Through functional expression screening, we identified a gene, designated Humanin (HN) cDNA, which encodes a short polypeptide and abolishes death of neuronal cells caused by multiple different types of familial Alzheimer's disease genes and by A beta amyloid, without effect on death by Q79 or superoxide dismutase-l mutants. Transfected HN cDNA was transcribed to the corresponding polypeptide and then was secreted into the cultured medium. The rescue action clearly depended on the primary structure of HN, This polypeptide would serve as a molecular clue for the development of new therapeutics for Alzheimer's disease targeting neuroprotection.